Wang Wei, Liu Quan-Jun, Cui Hong
College of Life Science, Henan Agricultural University, Zhengzhou, PR China.
Electrophoresis. 2007 Jul;28(14):2358-60. doi: 10.1002/elps.200600743.
Ammonium sulfate (AS) fractionation is often used in protein and enzyme purification; however, the resultant protein pellets have a high salt content and desalting by dialysis is required prior to next analysis. Here, we have developed a phenol-based method for quick desalting and concentrating proteins after AS fractionation of complex olive leaf protein extract. After redissolving, AS precipitates were desalted with phenol extraction and the abundance of beta-glucosidase in each fraction was monitored with a specific antibody. The results of electrophoresis and Western blot showed the feasibility of the method. The method is quick and universal, and does not need much technique.
硫酸铵（AS）分级分离常用于蛋白质和酶的纯化；然而，所得蛋白质沉淀含盐量高，在进行下一步分析之前需要通过透析进行脱盐。在此，我们开发了一种基于苯酚的方法，用于在对复杂的橄榄叶蛋白提取物进行AS分级分离后快速脱盐和浓缩蛋白质。重新溶解后，用苯酚萃取法对AS沉淀进行脱盐，并用特异性抗体监测各组分中β-葡萄糖苷酶的丰度。电泳和蛋白质印迹结果表明了该方法的可行性。该方法快速且通用，不需要太多技术。
