从叶片中或在大肠杆菌中表达后纯化核酮糖-1,5-二磷酸羧化酶/加氧酶激活酶。

Purification of Rubisco activase from leaves or after expression in Escherichia coli.

作者信息

Barta Csengele, Carmo-Silva A Elizabete, Salvucci Michael E

机构信息

U.S. Department of Agriculture, Arid-Land Agricultural Research Center, Maricopa, AZ, USA.

出版信息

Methods Mol Biol. 2011;684:363-74. doi: 10.1007/978-1-60761-925-3_28.

DOI:10.1007/978-1-60761-925-3_28

PMID:20960143

Abstract

Rubisco activase is a molecular chaperone that modulates the activation state of Rubisco by catalyzing the ATP-dependent removal of tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites. This chapter reports methods developed for the purification of native and recombinant Rubisco activase from leaves and bacterial cells, respectively.

摘要

核酮糖-1,5-二磷酸羧化酶/加氧酶激活酶是一种分子伴侣，它通过催化从核酮糖-1,5-二磷酸羧化酶/加氧酶催化位点上ATP依赖性去除紧密结合的抑制性糖磷酸来调节核酮糖-1,5-二磷酸羧化酶/加氧酶的激活状态。本章分别报道了从叶片和细菌细胞中纯化天然和重组核酮糖-1,5-二磷酸羧化酶/加氧酶激活酶所开发的方法。