Amatore Christian, Arbault Stéphane, Guille Manon, Lemaître Frédéric
Laboratoire PASTEUR, Ecole Normale Supérieure, CNRS, Université Pierre et Marie Curie-Paris 6, 24, rue Lhomond, 75231 Paris Cedex 05, France.
Chemphyschem. 2007 Aug 6;8(11):1597-605. doi: 10.1002/cphc.200700225.
Exocytosis is an important biological process used by cells to deliver messengers or effectors to target cells with high spatial, quantitative, and kinetic precision. This process occurs by interaction and fusion of vesicles containing the (bio)chemical information with the cell membrane to release their contents into the surrounding medium. Because of its importance for life, this mechanism underlies many biological controlling factors, including different families of proteins and enzymes. Tremendous efforts have been made over the last decade toward their determination. However, in parallel, many studies have also shown that the physical and chemical characteristics of the exocytosis actors (vesicle, membrane, and extracellular medium) could directly affect the quantitative or kinetic features of secretion. The major pieces evidence for this influence, which have been reported in the literature, are reviewed herein. It demonstrates undoubtedly that pure biological aspects cannot be segregated from the physicochemical context of living mechanisms.
胞吐作用是细胞用于将信使分子或效应分子以高空间、定量和动力学精度传递至靶细胞的重要生物学过程。该过程通过含有（生物）化学信息的囊泡与细胞膜相互作用并融合，将其内容物释放到周围介质中而发生。由于其对生命的重要性，这一机制是许多生物控制因素的基础，包括不同家族的蛋白质和酶。在过去十年中，人们为确定它们付出了巨大努力。然而，与此同时，许多研究也表明，胞吐作用参与者（囊泡、膜和细胞外介质）的物理和化学特性可能直接影响分泌的定量或动力学特征。本文综述了文献中报道的这种影响的主要证据。这无疑表明，纯粹的生物学方面不能与生命机制的物理化学背景相分离。
