使用4-甲基伞形酮基-α-D-葡萄糖苷和2-萘基-α-D-葡萄糖苷对α-D-葡萄糖苷酶进行微量化学研究（作者译）
[Microchemical investigation of alpha D-glucosidases using 4-methylumnelliferyl- and 2-naphthyl-alpha-d-glucoside (author's transl)].
作者信息
Gossrau R
出版信息
Histochemistry. 1977 May 20;52(2):187-97. doi: 10.1007/BF00492295.
In crude homogenates prepared from freeze-dried cryostate sections of various rat organs the Km and Vmax of acid and neutral alpha-glucosidase as well as the effect of the pH, substrate and enzyme concentration and the incubation time on the activity were determined fluorometrically with 4-methylumbelliferyl- and 2-naphthyl alpha-d-glucoside as substrates. On the basis of the biochemical data 2 assays were developed for the microchemical measurement of both alpha-glucosidases in groups of epithelial cells isolated from freeze dried cryostate sections of the epididymis, jejunum, ilium, liver and kidney of suckling and adult rats. The rate of hydrolysis of 2-naphthyl and 4-methylumbelliferyl alpha-d-glucoside differs moderately. However, due to the higher sensitivity of 4-methylumbelliferone the methylumbelliferyl derivative is preferable especially for the evaluation of alpha-d-glucosidases in cells with low enzyme activity.
在由各种大鼠器官的冻干冷冻切片制备的粗匀浆中，以4-甲基伞形酮基-α-D-葡萄糖苷和2-萘基-α-D-葡萄糖苷为底物，用荧光法测定了酸性和中性α-葡萄糖苷酶的Km和Vmax，以及pH、底物、酶浓度和孵育时间对活性的影响。基于这些生化数据，开发了两种测定方法，用于微量化学测定从哺乳期和成年大鼠的附睾、空肠、回肠、肝脏和肾脏的冻干冷冻切片中分离出的上皮细胞组中的两种α-葡萄糖苷酶。2-萘基-α-D-葡萄糖苷和4-甲基伞形酮基-α-D-葡萄糖苷的水解速率有适度差异。然而，由于4-甲基伞形酮的灵敏度更高，甲基伞形酮基衍生物更可取，特别是用于评估酶活性低的细胞中的α-D-葡萄糖苷酶。
Zotero 插件