Williamson D G, Gwilliam C, Layne D S
Can J Biochem. 1977 Jan;55(1):41-2. doi: 10.1139/o77-007.
Homogenates of liver and kidney tissue from young steers had beta-D-glucosidase (EC 3.2.1.21) activity toward 17alpha-estradiol-3-glucoside, 17beta-estradiol-3-glucoside, 17alpha-estradiol-17-glucoside, and deoxycorticosterone-21-glucoside. The activity towards the phenolic 3-glucosides was largely present in the 100 000 X g supernatant, while that towards 17alpha-estradiol-17-glucoside was concentrated in the microsomes. The use of beef liver preparations for the hydrolysis of steroid 'glucuronide' fractions could result in hydrolysis of other steroid glycosides which might be present.
来自幼年公牛的肝脏和肾脏组织匀浆对17α-雌二醇-3-葡萄糖苷、17β-雌二醇-3-葡萄糖苷、17α-雌二醇-17-葡萄糖苷和脱氧皮质酮-21-葡萄糖苷具有β-D-葡萄糖苷酶（EC 3.2.1.21）活性。对酚类3-葡萄糖苷的活性主要存在于100000×g上清液中，而对17α-雌二醇-17-葡萄糖苷的活性则集中在微粒体中。使用牛肝制剂水解类固醇“葡萄糖醛酸苷”部分可能会导致其他可能存在的类固醇糖苷水解。
