Herst Patries M, Hesketh Elizabeth L, Ritchie David S, Berridge Michael V
Malaghan Institute of Medical Research, P.O. Box 7060, Wellington, New Zealand.
Leuk Res. 2008 Feb;32(2):327-33. doi: 10.1016/j.leukres.2007.04.014. Epub 2007 Jun 18.
Glycolytic cancers are resistant to many forms of chemotherapy and some respond poorly to differentiation therapies. Here, we investigate the effects of exposure to all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) on differentiation and cell survival in the human leukemia cell line, HL60 and its mitochondrial gene knockout mutant, HL60rho0. Glycolytic HL60rho0 cells exposed to single and combined treatments expressed less CD15, in most cases, but produced a stronger respiratory burst than parental HL60 cells. HL60rho0 cells were also significantly more resistant to apoptosis after combined ATO+ATRA treatment compared with HL60 cells, and this was associated with failure to upregulate Fas expression.
糖酵解型癌症对多种化疗形式具有抗性，并且有些对分化疗法反应不佳。在此，我们研究了全反式维甲酸（ATRA）和三氧化二砷（ATO）对人白血病细胞系HL60及其线粒体基因敲除突变体HL60rho0的分化和细胞存活的影响。在大多数情况下，暴露于单一和联合处理的糖酵解型HL60rho0细胞表达的CD15较少，但比亲代HL60细胞产生更强的呼吸爆发。与HL60细胞相比，ATO + ATRA联合处理后，HL60rho0细胞对凋亡的抗性也显著更强，这与未能上调Fas表达有关。
Zotero 插件