人脐带源干细胞在缺血性疾病中的治疗潜力

Therapeutic potential of human umbilical cord-derived stem cells in ischemic diseases.

作者信息

Wu K H, Zhou B, Mo X M, Cui B, Yu C T, Lu S H, Han Z C, Liu Y L

机构信息

Pediatric Cardiac Center, Department of Surgery, Cardiovascular Institute and Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

出版信息

Transplant Proc. 2007 Jun;39(5):1620-2. doi: 10.1016/j.transproceed.2006.12.041.

DOI:10.1016/j.transproceed.2006.12.041

PMID:17580202

Abstract

Recent advances suggest human umbilical cord is a new source for stem cells. Our laboratory has established a method to readily isolate and expand stem cells from human umbilical cord tissues. The aim of this study was to investigate the therapeutic potential of human umbilical cord-derived stem (UCDS) cells in ischemic diseases. The UCDS cells were characterized by flow cytometry and differentiation into osteogenic and adipogenic cells. Unilateral hind limb ischemia was surgically induced by femoral artery ligation in nude mice. The animals were intramuscularly injected with 10(6) UCDS cells or control phosphate-buffered saline. Blood perfusion of ischemic limbs was detected by laser Doppler perfusion imaging. Transplantation of UCDS cells to the ischemic limbs of nude mice significantly improved the blood flow to the affected limbs. Thus, transplantation of UCDS cells may potentially be a promising treatment for human ischemic diseases.

摘要

近期进展表明，人脐带是干细胞的一个新来源。我们实验室已建立了一种从人脐带组织中轻松分离和扩增干细胞的方法。本研究的目的是探讨人脐带源干细胞（UCDS）在缺血性疾病中的治疗潜力。通过流式细胞术以及向成骨细胞和脂肪生成细胞的分化对UCDS细胞进行了表征。通过在裸鼠中结扎股动脉手术诱导单侧后肢缺血。给动物肌肉注射10(6)个UCDS细胞或对照磷酸盐缓冲盐水。通过激光多普勒灌注成像检测缺血肢体的血液灌注。将UCDS细胞移植到裸鼠的缺血肢体可显著改善患侧肢体的血流。因此，UCDS细胞移植可能是治疗人类缺血性疾病的一种有前景的方法。