Qian Wei, Ni Jinzhi, Luo Yongming, Li Xiuhua, Zou Dexun
State Key Laboratory of Soil and Sustainable Agriculture, Soil and Environment Bioremediation Research Center, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Se Pu. 2007 Mar;25(2):221-5.
Polycyclic aromatic hydrocarbons (PAHs) are the environmental organic pollutants which can induce cancers, teratogeny and organism saltation. Therefore, a reliable detection method is essential for studying the environmentally hazardous PAHs. The high performance liquid chromatography with a highly sensitive fluorescence detector was introduced to determine 15 PAHs of the priority pollutants named by the US Environmental Protection Agency (EPA) in soil. The gradient elution procedure was improved and the detection wavelength was optimized. The detection limits and the recoveries of the improved method for the 15 PAHs were in the ranges of 0. 12 microg/kg to 1. 57 microLg/kg and 73% to 126%, respectively. The relative standard deviations of this method were in the range of 0. 53% to 3. 57%. The results indicated that this method is a reliable detection method with low detection limit, high sensitivity and repeatability for the determination of PAHs in soil.
多环芳烃（PAHs）是能够诱发癌症、致畸和生物体突变的环境有机污染物。因此，一种可靠的检测方法对于研究具有环境危害性的多环芳烃至关重要。采用配备高灵敏度荧光检测器的高效液相色谱法测定土壤中美国环境保护局（EPA）指定的15种优先污染物多环芳烃。改进了梯度洗脱程序并优化了检测波长。改进方法对15种多环芳烃的检测限在0.12微克/千克至1.57微克/千克范围内，回收率在73%至126%之间。该方法的相对标准偏差在0.53%至3.57%范围内。结果表明，该方法是一种可靠的检测方法，用于测定土壤中的多环芳烃时具有低检测限、高灵敏度和可重复性。
