Li Cheuk Fai, Wong Jonathan W C, Huie Carmen W, Choi Martin M F
Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, China.
J Chromatogr A. 2008 Dec 19;1214(1-2):11-6. doi: 10.1016/j.chroma.2008.10.062. Epub 2008 Oct 21.
Cloud point methodology has been used to develop a novel preconcentration and an analytical method for polycyclic aromatic hydrocarbons (PAHs) in soil sample. The nonionic surfactant Tergitol 15-S-7 was successfully used as the surfactant-mediated extractant in both ultrasonic and microwave-assisted extractions. Over 90% of recoveries for various PAHs in soil were obtained under the optimal experimental conditions. The extracts were then preconcentrated and analyzed by our proposed on-line coupling method "flow injection-cloud point preconcentration-high-performance liquid chromatography" (FI-CPP-HPLC) equipped with a fluorescence detector and an excitation/emission wavelength program. The preconcentration system and optimal working conditions were established. The limit of detection of the FI-CPE-HPLC system ranges from 0.101 to 0.456 microg/L for the selected PAHs, i.e., phenanthrene, pyrene, chrysene, benzo[k]fluoranthene and benzo[a]pyrene. Our proposed technique provides a reliable, simple and automatic analytical method for the determination of PAHs in environmental soil samples.
浊点法已被用于开发一种用于土壤样品中多环芳烃（PAHs）的新型预浓缩和分析方法。非离子表面活性剂Tergitol 15-S-7在超声和微波辅助萃取中均成功用作表面活性剂介导的萃取剂。在最佳实验条件下，土壤中各种PAHs的回收率超过90%。然后，通过我们提出的在线联用方法“流动注射-浊点预浓缩-高效液相色谱法”（FI-CPP-HPLC）对提取物进行预浓缩和分析，该方法配备了荧光检测器和激发/发射波长程序。建立了预浓缩系统和最佳工作条件。对于选定的PAHs，即菲、芘、屈、苯并[k]荧蒽和苯并[a]芘，FI-CPE-HPLC系统的检测限范围为0.101至0.456μg/L。我们提出的技术为环境土壤样品中PAHs的测定提供了一种可靠、简单且自动的分析方法。
