Lange Mette, Vincze Eva, Wieser Herbert, Schjoerring Jan K, Holm Preben B
Faculty of Agricultural Sciences, Department of Genetics and Biotechnology, Research Centre Flakkebjerg, University of Aarhus, Forsoegsvej 1, DK-4200 Slagelse, Denmark.
J Agric Food Chem. 2007 Jul 25;55(15):6074-81. doi: 10.1021/jf0709505. Epub 2007 Jun 20.
Barley has for feeding purposes a shortage of essential amino acids, especially lysine, threonine, and methionine, and an excess of proline and glutamine. In the present study, we have introduced into barley an antisense construct against C-hordeins, the storage protein with the lowest nutritional quality. SDS-PAGE and reverse phase HPLC revealed a relative reduction in the amounts of C-hordeins and relative increases in the content of the other storage proteins. The five different lines analyzed had lower amounts of proline, glutamic acid/glutamine, and phenylalanine (up to 12%, 6%, and 9% reductions), while the lysine, threonine, and methionine content was increased with up to 16%, 13% and 11%. It is concluded that antisense mediated suppression of C-hordein synthesis may be a promising approach for improving the nutritional value of barley as a feed crop while at the same time reducing the environmental nitrogen load.
大麦作为饲料使用时，必需氨基酸含量不足，尤其是赖氨酸、苏氨酸和蛋氨酸，而脯氨酸和谷氨酰胺含量过高。在本研究中，我们将针对C-醇溶蛋白（营养品质最低的贮藏蛋白）的反义构建体导入大麦。SDS-PAGE和反相HPLC分析表明，C-醇溶蛋白的含量相对降低，而其他贮藏蛋白的含量相对增加。分析的五个不同株系中，脯氨酸、谷氨酸/谷氨酰胺和苯丙氨酸的含量较低（分别降低了12%、6%和9%），而赖氨酸、苏氨酸和蛋氨酸的含量分别增加了16%、13%和11%。研究得出结论，反义介导的C-醇溶蛋白合成抑制可能是一种有前景的方法，既能提高大麦作为饲料作物的营养价值，又能同时降低环境氮负荷。
