通心络超微粉对高脂饮食喂养家兔主动脉中核因子-κB、细胞间黏附分子-1及血管细胞黏附分子-1表达的影响
[The effects of Tongxinluo Supermicro Powder on nuclear factor-kappaB, intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in aorta of rabbits fed with high-lipid diet].
作者信息
Wu Yi-ling, You Jia-hua, Yuan Guo-qiang, Liang Jun-qing, Jia Zhen-hua, Liu Ke-jian, Wei Cong
机构信息
The Pharmaceutical Research Institute of Hebei Medicine University, Hebei Shijiazhuang 050035, China.
出版信息
Zhonghua Xin Xue Guan Bing Za Zhi. 2007 Mar;35(3):271-4.
OBJECTIVE
To observe the effects of Tongxinluo Supermicro Powder on the nuclear factor-kappaB (NF-kappaB), inter-cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in aorta of rabbits fed with high-lipid diet.
METHODS
Healthy male New Zealand rabbits were randomly divided into 4 groups (n = 8 each): control group, model group, atorvastatin group (3 mg x kg(-1) x d(-1) per gavage), and Tongxinluo group (0.31 g x kg(-1) x d(-1) per gavage). At the end of 6 weeks, the expression of NF-kappaB, ICAM-1 and VCAM-1 were observed by immunochemistry methods, Western blotting and reverse transcription polymerase chain reaction (RT-PCR).
RESULT
The nuclear translocation of NF-kappaB in aortic endothelial cells and the gene expressions of NF-kappaB, ICAM-1 and VCAM-1 at protein and mRNA levels of the model group was significantly increased compared that in the control group (all P < 0.05), these effects could be significantly attenuated by atorvastatin and Tongxinluo Supermicro Powder (P < 0.01 vs. model group).
CONCLUSIONS
Similar as atorvastatin, Tongxinluo Supermicro Powder could relieve the process of atherosclerosis by decreasing the nuclear translocation of NF-kappaB and reducing the expression of ICAM-1, VCAM-1 in this model.
目的
观察通心络超微粉对高脂饮食喂养家兔主动脉中核因子-κB（NF-κB）、细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）表达的影响。
方法
将健康雄性新西兰家兔随机分为4组（每组n = 8）：对照组、模型组、阿托伐他汀组（灌胃剂量为3 mg·kg⁻¹·d⁻¹）和通心络组（灌胃剂量为0.31 g·kg⁻¹·d⁻¹）。6周结束时，采用免疫化学方法、蛋白质印迹法和逆转录聚合酶链反应（RT-PCR）观察NF-κB、ICAM-1和VCAM-1的表达。
结果
与对照组相比，模型组主动脉内皮细胞中NF-κB的核转位以及NF-κB、ICAM-1和VCAM-1在蛋白质和mRNA水平的基因表达均显著增加（均P < 0.05），阿托伐他汀和通心络超微粉可显著减弱这些作用（与模型组相比，P < 0.01）。
结论
与阿托伐他汀相似，通心络超微粉在该模型中可通过减少NF-κB的核转位以及降低ICAM-1、VCAM-1的表达来缓解动脉粥样硬化进程。
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