Laboratory for Experimental Surgery and Surgical Research N.S. Christeas, Athens University Medical School, Athens, Greece.
In Vivo. 2012 Mar-Apr;26(2):243-50.
Members of the immunoglobulin superfamily of endothelial adhesion molecules, vascular cell adhesion molecule (VCAM-1) and intercellular cell adhesion molecule (ICAM-1), participate in leukocyte adhesion to the endothelium and play an important role in all stages of atherosclerosis. The aim of the study was to examine the expression of VCAM-1 and ICAM-1 in the aorta of rats at the early stages of atherosclerosis and the correlation with their plasma concentrations.
Male rats (n=44), 10 weeks of age, were divided in 4 groups. Groups A and C (n=12) were fed with rich cholesterol diet for 12 and 16 weeks, respectively. Group B (regression group, n=12) was fed for the first 12 weeks with rich cholesterol diet and for another 4 weeks with normal diet. Group D (control group, n=8) was fed with normal diet for 12 weeks. We measured the serum lipid profile, the concentration of soluble ICAM-1 and the immunohistochemical expression of ICAM-1 and VCAM-1 in the endothelium, media and vasa vasorum of the aorta.
There were significant differences (p<0.05) in the expression of ICAM-1 between group C (maximum time of rich cholesterol diet) and all other groups in the 3 groups of the aorta studied. There was regression of the expression of ICAM-1 in group B and significant differences (p<0.05) between group B and all the other groups, except group D in the expression of ICAM-1. There were no significant differences in the expression of VCAM-1 between any groups. The serum concentration of soluble ICAM-1 positively correlated with the expression of the molecule in the vasa vasorum (r=0.35, p<0.05) and fibroblasts/smooth muscular cells (r=0.34, p<0.05) of the aorta.
A cholesterol diet plays a role in the expression of ICAM-1 but not in that of VCAM-1 in the rat aorta. The expression of ICAM-1 in the aorta regresses after the withdrawal of a cholesterol-rich diet. Soluble ICAM-1 is a reliable measure of ICAM-1 expression in the aorta, vasa vasorum and fibroblasts/smooth muscle cells.
免疫球蛋白超家族的内皮细胞黏附分子成员，血管细胞黏附分子（VCAM-1）和细胞间黏附分子（ICAM-1），参与白细胞黏附于内皮细胞，并在动脉粥样硬化的所有阶段发挥重要作用。本研究的目的是检测在动脉粥样硬化早期大鼠主动脉中 VCAM-1 和 ICAM-1 的表达及其与血浆浓度的相关性。
10 周龄雄性大鼠（n=44）分为 4 组。A 组和 C 组（n=12）分别给予富含胆固醇饮食 12 周和 16 周。B 组（回归组，n=12）前 12 周给予富含胆固醇饮食，后 4 周给予普通饮食。D 组（对照组，n=8）给予普通饮食 12 周。我们测量了血清脂质谱、可溶性 ICAM-1 浓度以及主动脉内皮、中膜和血管腔的 ICAM-1 和 VCAM-1 的免疫组化表达。
在研究的 3 组主动脉中，C 组（给予富含胆固醇饮食的最长时间）与其他所有组之间的 ICAM-1 表达均有显著差异（p<0.05）。B 组的 ICAM-1 表达有回归，B 组与除 D 组以外的所有组之间的 ICAM-1 表达均有显著差异（p<0.05）。各组间 VCAM-1 表达无显著差异。血清可溶性 ICAM-1 浓度与主动脉血管腔（r=0.35，p<0.05）和纤维母细胞/平滑肌细胞（r=0.34，p<0.05）中分子的表达呈正相关。
胆固醇饮食在大鼠主动脉中 ICAM-1 的表达中起作用，但对 VCAM-1 的表达不起作用。富含胆固醇饮食停止后，主动脉 ICAM-1 的表达会消退。可溶性 ICAM-1 是主动脉、血管腔和纤维母细胞/平滑肌细胞中 ICAM-1 表达的可靠指标。
