Wen Aidong, Wang Zhipeng, Hang Taijun, Jia Yanyan, Zhang Tingting, Wu Yin, Gao Xuan, Yang Zhifu
Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):348-52. doi: 10.1016/j.jchromb.2007.05.014. Epub 2007 May 26.
A simple, rapid, sensitive and reliable high performance liquid chromatography (HPLC) method for the determination of the anti-ulcer drug sofalcone in human plasma was developed. Plasma was extracted with ethyl acetate under acidic conditions and sofalcone was determined by HPLC using a C18 column and (methanol-0.1% formic acid aqueous 80:20) mobile phase. The linear calibration curves of sofalcone in human plasma were obtained over the concentration range of 0.01-5.0 microg/ml. The lower limit of quantitation (LLOQ) was 10 ng/ml in human plasma. The precision measured for plasma was within 15%. Extraction recovery was over 85% in blood. The method was successfully applied to the identification and quantification of sofalcone in pharmacokinetic studies.
