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利用来自茂原链霉菌的青霉素V酰基转移酶催化的酰基转移反应进行β-内酰胺抗生素和N-脂肪酰化氨基化合物的酶促合成。

Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis.

作者信息

Koreishi Mayuko, Tani Kazuha, Ise Yuuichi, Imanaka Hiroyuki, Imamura Koreyoshi, Nakanishi Kazuhiro

机构信息

Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Japan.

出版信息

Biosci Biotechnol Biochem. 2007 Jun;71(6):1582-6. doi: 10.1271/bbb.70052.

DOI:10.1271/bbb.70052
PMID:17587696
Abstract

Penicillin V acylase from Streptomyces mobaraensis (Sm-PVA) showed high acyl-transfer activity in reactions using methyl esters of carboxylic acid (acyl donor) and amino compounds (nucleophile), to produce the corresponding amides. Moreover, Sm-PVA had broad substrate specificity, as indicated by the fact that it catalyzed the efficient synthesis of beta-lactam antibiotics, capsaicin derivatives, and N-fatty-acyl-amino acid/N-fatty-acyl-peptide derivatives.

摘要

来自茂原链霉菌的青霉素V酰基转移酶（Sm-PVA）在使用羧酸甲酯（酰基供体）和氨基化合物（亲核试剂）的反应中表现出高酰基转移活性，以产生相应的酰胺。此外，Sm-PVA具有广泛的底物特异性，这一事实表明它能催化高效合成β-内酰胺抗生素、辣椒素衍生物和N-脂肪酰基氨基酸/N-脂肪酰基肽衍生物。

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Enzymatic synthesis of beta-lactam antibiotics and N-fatty-acylated amino compounds by the acyl-transfer reaction catalyzed by penicillin V acylase from Streptomyces mobaraensis.利用来自茂原链霉菌的青霉素V酰基转移酶催化的酰基转移反应进行β-内酰胺抗生素和N-脂肪酰化氨基化合物的酶促合成。
Biosci Biotechnol Biochem. 2007 Jun;71(6):1582-6. doi: 10.1271/bbb.70052.
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