Williams Elizabeth A, Perkins Susan N, Smith Nicole C P, Hursting Stephen D, Lane Michelle A
Division of Nutritional Sciences, Department of Human Ecology at the University of Texas at Austin, Austin, TX 78712, USA.
Ann Nutr Metab. 2007;51(3):232-43. doi: 10.1159/000104143. Epub 2007 Jun 18.
BACKGROUND/AIMS: To compare weight loss, body composition, and metabolic changes in response to carbohydrate versus dietary energy restriction (DER) in obese mice.
One hundred C57BL/6 mice were randomized into five groups of 20. The group of high-carbohydrate (HC) mice consumed an HC diet ad libitum and the group of high-fat (HF) mice consumed an HF diet ad libitum for 14 weeks. Additional groups consumed the HF diet for 7 weeks ad libitum and during weeks 8-14 were switched to either a low-carbohydrate diet (LC) consumed ad libitum, the HC diet pair-fed (PF) to the energy intake of the LC group, or an HC DER regimen providing 70% of the energy intake of the HF group.
At 14 weeks, the LC and HF groups weighed more and exhibited higher percent fat mass and lower bone mineral density than the HC, PF, and DER groups. Relative to the DER group, the LC group displayed comparable serum ketone bodies but higher serum glucose, triglycerides, cholesterol, leptin, insulin, and insulin-like growth factor-1.
In contrast to DER, the LC diet did not cause weight loss or reduce serum markers associated with obesity-related diseases other than diabetes in obese mice, suggesting that carbohydraterestriction without reduced energy intake does not induce weight loss.
背景/目的：比较肥胖小鼠在碳水化合物限制与饮食能量限制（DER）情况下的体重减轻、身体成分及代谢变化。
将100只C57BL/6小鼠随机分为五组，每组20只。高碳水化合物（HC）组小鼠随意进食HC饮食，高脂肪（HF）组小鼠随意进食HF饮食，持续14周。其他组小鼠随意进食HF饮食7周，在第8 - 14周改为随意进食低碳水化合物饮食（LC）、将HC饮食配对喂养（PF）至LC组的能量摄入量，或采用提供HF组能量摄入量70%的HC DER方案。
14周时，LC组和HF组的体重更高，脂肪量百分比更高，骨矿物质密度低于HC组、PF组和DER组。相对于DER组，LC组的血清酮体水平相当，但血清葡萄糖、甘油三酯、胆固醇、瘦素、胰岛素和胰岛素样生长因子-1水平更高。
与DER不同，LC饮食未导致肥胖小鼠体重减轻，也未降低除糖尿病外与肥胖相关疾病相关的血清标志物，提示不减少能量摄入的碳水化合物限制不会引起体重减轻。
