Davidovitch Z, Montgomery P C, Eckerdal O, Gustafson G T
Calcif Tissue Res. 1976 Mar 31;19(4):305-15. doi: 10.1007/BF02564013.
By utilizing immunohistochemical procedures it was possible to locate bone cells that contained adenosine 3'5'-monophosphate (cyclic AMP) in frozen, undecalcified cat jaw sections. An immunoglobulin-enzyme bridge method was found to be superior to other techniques, including immunofluorescence. Differences in staining pattern were found between bone cells of kittens and young adult cats, indicating different metabolic levels related to cellular maturity, and different intracellular compartmentalization of cyclic AMP in the two age groups.
通过利用免疫组织化学方法，能够在冷冻的、未脱钙的猫下颌骨切片中定位含有3',5'-环磷酸腺苷（环磷酸腺苷）的骨细胞。发现免疫球蛋白-酶桥法优于其他技术，包括免疫荧光法。在小猫和成年猫的骨细胞之间发现了染色模式的差异，这表明与细胞成熟度相关的代谢水平不同，并且两个年龄组中环磷酸腺苷的细胞内分隔也不同。
