Bloom F E, Hoffer B J, Battenberg E R, Siggins G R, Steiner A L, Parker C W, Wedner H J
Science. 1972 Aug 4;177(4047):436-8. doi: 10.1126/science.177.4047.436.
Adenosine 3',5'-monophosphate is localized in specific cerebellar neurons, as shown by fluorescence immunocytochemistry with a specific rabbit immunoglobulin. Positive staining is exhibited by Purkinje neurons and granule cells. The increase in concentration of cyclic adenosine monophosphate in the cerebellum, which is known to follow decapitation, is represented by greatly increased fluorescence of Purkinje neurons only. These immunofluorescence data provide the first evidence for localization of cyclic adenosine monophosphate in specific neurons and may permit further exploration into the role of this cyclic nucleotide in neuronal function.
通过使用特异性兔免疫球蛋白的荧光免疫细胞化学方法显示，3',5'-环磷酸腺苷定位于特定的小脑神经元中。浦肯野神经元和颗粒细胞呈现阳性染色。已知断头后小脑中环磷酸腺苷浓度会增加，这仅表现为浦肯野神经元荧光大幅增强。这些免疫荧光数据为环磷酸腺苷在特定神经元中的定位提供了首个证据，并可能有助于进一步探索这种环核苷酸在神经元功能中的作用。
