Xu Meiying, Guo Jun, Sun Guoping
Guangdong Institute of Microbiology, Guangzhou, 510070, People's Republic of China.
Appl Microbiol Biotechnol. 2007 Sep;76(3):719-26. doi: 10.1007/s00253-007-1032-7. Epub 2007 Jun 23.
The complete biodegradation of azo dye, Fast Acid Red GR, was observed under microaerophilic conditions by Shewanella decolorationis S12. Although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing products can only be achieved under microaerophilic conditions. Under microaerophilic conditions, S. decolorationis S12 could use a range of carbon sources for azo dye decolorization, including lactate, formate, glucose and sucrose, with lactate being the optimal carbon source. Sulfonated aromatic amines were not detected during the biotransformation of Fast Acid Red GR, while H(2)S formed. The decolorizing products, aniline, 1,4-diaminobenzene and 1-amino-2-naphthol, were followed by complete biodegradation through catechol and 4-aminobenzoic acid based on the analysis results of GC-MS and HPLC.
嗜冷栖热袍菌S12在微需氧条件下可实现偶氮染料酸性红GR的完全生物降解。虽然在厌氧条件下测得的脱色率最高，在好氧条件下获得的生物量最高，但只有在微需氧条件下才能实现脱色产物的进一步生物降解。在微需氧条件下，嗜冷栖热袍菌S12可利用多种碳源进行偶氮染料脱色，包括乳酸盐、甲酸盐、葡萄糖和蔗糖，其中乳酸盐是最佳碳源。在酸性红GR的生物转化过程中未检测到磺化芳香胺，但生成了H₂S。根据GC-MS和HPLC的分析结果，脱色产物苯胺、1,4-二氨基苯和1-氨基-2-萘酚通过邻苯二酚和4-氨基苯甲酸被完全生物降解。
