自养和异养氧化亚铁硫杆菌中无机硫代谢的酶学比较

Enzymic comparisons of the inorganic sulfur metabolism in autotrophic and heterotrophic Thiobacillus ferrooxidans.

作者信息

Tuovinen P H, Kelley B C, Nicholas D J

出版信息

Can J Microbiol. 1976 Jan;22(1):109-13. doi: 10.1139/m76-016.

Abstract

Activities of enzymes which mediate the oxidation of thiosulfate to sulfate and the assimilation of sulfate to sulfide were assayed in various cell-free fractions of Thiobacillus ferrooxidans grown autotrophically on either ferrous iron or thiosulfate or heterotrophically on glucose. There was no activity of the thiosulfate-oxidizing enzyme in extracts of bacteria grown with ferrous iron. Comparable activities for ATP-sulfurylase (EC 2.7.7.4), ADP-sulfurylase (EC 2.7.7.5), and adenylate kinase (EC 2.7.4.3) were found in the bacteria grown autotrophically with either Fe2+ or S2O32- or heterotrophically with glucose.

摘要

在以亚铁离子或硫代硫酸盐为自养生长底物，或以葡萄糖为异养生长底物的氧化亚铁硫杆菌的各种无细胞组分中，测定了介导硫代硫酸盐氧化为硫酸盐以及硫酸盐同化生成硫化物的酶的活性。以亚铁离子生长的细菌提取物中没有硫代硫酸盐氧化酶的活性。在以Fe2+或S2O32-自养生长或以葡萄糖异养生长的细菌中，发现了ATP硫酸化酶（EC 2.7.7.4）、ADP硫酸化酶（EC 2.7.7.5）和腺苷酸激酶（EC 2.7.4.3）的可比活性。