来自硫杆菌A2的硫氰酸酶：通过质子核磁共振光谱法测定活性
Rhodanese from Thiobacillus A2: determination of activity by proton nuclear magnetic resonance spectroscopy.
作者信息
Silver M, Howarth O W, Kelly D P
出版信息
J Gen Microbiol. 1976 Dec;97(2):285-8. doi: 10.1099/00221287-97-2-285.
PMID:796411
Abstract
Rhodanese from Thiobacillus A2 was shown by proton nuclear magnetic resonance (NMR) spectroscopy to use dihydrolipoate or dihydrolipoamide as acceptor of the sulphane moiety of thiosulphate with the formation of alpha-lipoate or lipoamide respectively. Correlation is shown between assays of the enzyme activity by NMR spectroscopy and by ultraviolet spectrophotometry.
摘要
通过质子核磁共振（NMR）光谱法表明，来自硫杆菌A2的硫氰酸酶使用二氢硫辛酸或二氢硫辛酰胺作为硫代硫酸盐硫烷部分的受体，分别形成α-硫辛酸或硫辛酰胺。通过NMR光谱法和紫外分光光度法对酶活性的测定之间显示出相关性。
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