小鼠发育大脑中细胞增殖和分化的变化。
Changes of cell proliferation and differentiation in the developing brain of mouse.
作者信息
Qiu Lin, Zhu Chang-Lian, Wang Xiao-Yang, Xu Fa-Lin
机构信息
Department of Anesthesia, Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
出版信息
Neurosci Bull. 2007 Jan;23(1):46-52. doi: 10.1007/s12264-007-0007-0.
OBJECTIVE
To investigate the cell proliferation and differentiation in the developing brain of mouse.
METHODS
C57/BL6 mice were divided into 3 groups at random. Bromodeoxyuridine (BrdU) was injected into the brains in different development periods once a day for 7 d. The brains were retrieved 4 weeks after the last BrdU injection. Immunohistochemical and immunofluorescent studies were carried out for detecting cell proliferation (BrdU) and cell differentiation (NeuN, APC, Iba1, and S100beta), respectively.
RESULTS
The number of BrdU labeled cells decreased significantly with the development of the brain. Cell proliferation was prominent in the cortex and striatum. A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.
CONCLUSION
These results demonstrated that cell proliferation, differentiation and survival were age and brain region related.
目的
研究小鼠发育中大脑的细胞增殖与分化。
方法
将C57/BL6小鼠随机分为3组。在不同发育时期每天向大脑注射一次溴脱氧尿苷（BrdU），共注射7天。在最后一次注射BrdU后4周取出大脑。分别进行免疫组织化学和免疫荧光研究以检测细胞增殖（BrdU）和细胞分化（NeuN、APC、Iba1和S100β）。
结果
随着大脑发育，BrdU标记的细胞数量显著减少。细胞增殖在皮质和纹状体中较为突出。在发育早期的皮质以及所有组的纹状体和海马CA区可检测到一小部分BrdU和NeuN双标记细胞。大多数BrdU标记的细胞是神经胶质细胞，随着大脑成熟，神经胶质细胞数量急剧减少。神经发生是齿状回中的主要细胞生成方式。
结论
这些结果表明细胞增殖、分化和存活与年龄及脑区有关。
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