Mischke R, Nolte I
Klinik für kleine Haustiere, Tierärztlichen Hochschule Hannover.
Tierarztl Prax. 1991 Oct;19(5):561-6.
When the global coagulation tests Quick's test, aPTT and thrombin time are carried out on dogs using reagents from the human medical sector according to the manufacturer's instructions, they react insensitively to deficiencies of the corresponding coagulation factors or to the presence of fibrin degradation products. In the present study it was attempted to develop sensitive global tests for the dog by modifying these tests. The Quick's test reacted apparently more sensitively after a 1:4 dilution of the test plasma with physiological saline solution. The sensitivity of the aPTT was demonstrated by the addition of BaSO4-adsorbed plasma to normal plasma. It seems to be particularly advantageous to combine a 1:3 plasma dilution with a reduction in the CaCl2-concentration to 5 mmol/l. Likewise, plasma dilution allows the thrombin time to react more sensitively in the presence of increased concentrations of fibrin degradation products. The aPTT and thrombin time are, as in human beings, suited for the control of a heparin therapy. However, since prolongation of the coagulation times is considerably dependent on the test chosen, the heparin sensitivity of the test should be determined in vitro prior to use.
当按照制造商的说明使用人类医疗领域的试剂对犬进行全球凝血试验（奎克试验、活化部分凝血活酶时间（aPTT）和凝血酶时间）时，它们对相应凝血因子缺乏或纤维蛋白降解产物的存在反应不敏感。在本研究中，试图通过修改这些试验来开发针对犬的敏感全球试验。用生理盐水将试验血浆稀释1:4后，奎克试验的反应明显更敏感。通过向正常血浆中添加硫酸钡吸附血浆证明了aPTT的敏感性。将1:3血浆稀释与将氯化钙浓度降低至5 mmol/L相结合似乎特别有利。同样，血浆稀释可使凝血酶时间在纤维蛋白降解产物浓度增加时反应更敏感。与人类一样，aPTT和凝血酶时间适用于肝素治疗的监测。然而，由于凝血时间的延长在很大程度上取决于所选择的试验，因此在使用前应在体外确定试验的肝素敏感性。
