[监测金黄色葡萄球菌菌株中编码杀白细胞素的基因]
[Monitoring genes encoding Panton-Valentine leukocidin in Staphylococcus aureus strains].
作者信息
Machová I, Petrás P, Blazková E, Knapová L
机构信息
Státní zdravotní ústav, Centrum epidemiologie a mikrobiologie, Národní referencní laborator pro stafylokoky, Praha.
出版信息
Epidemiol Mikrobiol Imunol. 2007 Apr;56(2):88-93.
STUDY OBJECTIVES
To detect the genes encoding an important virulence factor, Panton-Valentine leukocidin, in S. aureus isolates from clinical specimens.
MATERIAL AND METHODS
S. aureus strains from clinical specimens, mainly from patients with skin diseases, referred by microbiological laboratories of the Czech Republic. The strains were identified by both conventional phenotyping methods and molecular biological procedures, in particular polymerase chain reaction.
RESULTS
Altogether 108 (8.1%) of 1336 S. aureus strains had the genes encoding Panton-Valentine leukocidin in DNA. Only 11 of these strains were MRSA.
CONCLUSIONS
S. aureus strains producing Panton-Valentine leukocidin play an important role in serious infections, particularly of the skin. NRL for Staphylococci, National Institute of Public Health, Centre of Epidemiology and Microbiology, is able to detect the production of this toxin, under optimal conditions, within two days.
研究目的
检测临床标本中分离出的金黄色葡萄球菌菌株中编码重要毒力因子——杀白细胞素（Panton-Valentine leukocidin，PVL）的基因。
材料与方法
从捷克共和国微生物实验室送检的临床标本中获取金黄色葡萄球菌菌株，主要来自皮肤病患者。通过传统表型分析方法和分子生物学程序，特别是聚合酶链反应对菌株进行鉴定。
结果
1336株金黄色葡萄球菌中，共有108株（8.1%）在DNA中含有编码杀白细胞素的基因。其中只有11株为耐甲氧西林金黄色葡萄球菌（MRSA）。
结论
产生杀白细胞素的金黄色葡萄球菌菌株在严重感染，尤其是皮肤感染中起重要作用。国家公共卫生研究所、流行病学和微生物学中心的葡萄球菌国家参考实验室（NRL）能够在最佳条件下于两天内检测出该毒素的产生。
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