Biotechnology and Bioengineering Central Research Laboratory, Izmir Institute of Technology, Gulbahce Campus, Urla, 35430 Izmir, Turkey.
Int J Food Microbiol. 2010 Apr 15;138(3):287-91. doi: 10.1016/j.ijfoodmicro.2010.01.023. Epub 2010 Jan 25.
In this study, three Panton-Valentine Leukocidin gene carrying methicillin-susceptible Staphylococcus aureus (MSSA) strains (M1-AAG42B, PY30C-b and YF1B-b) were isolated from different food samples in Kesan-Edirne, Turkey. These strains were characterized on the basis of MLST type, spa type, virulence factor gene contents, antibiotic susceptibilities against 21 antibiotics and biofilm formation. The genetic relatedness of the strains was determined by PFGE. In addition, the complete gene sequences of lukS-PV and lukF-PV were also investigated. All strains were found to be susceptible to tested antibiotics and they were mecA negative. Three strains showed the same PFGE band pattern, ST152 clonal type and t355 spa type. In the detection of virulence factor genes, sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq, seu, eta, etb, set1, geh and tst genes were not detected. All strains showed the positive results for alpha- and beta-haemolysin genes (hla and hlb), protease encoding genes (sspA, sspB and aur), lukE and lukD leukocidin genes (lukED). The strains were found to be non-biofilm formers. By this study, the virulence properties of the strains were described and this is one of the first reports regarding PVL-positive MSSA strains from food.
在这项研究中，从土耳其 Kesan-Edirne 的不同食品样本中分离出了三株携带 Panton-Valentine 白细胞毒素基因的耐甲氧西林金黄色葡萄球菌（MSSA）菌株（M1-AAG42B、PY30C-b 和 YF1B-b）。这些菌株基于 MLST 型、spa 型、毒力因子基因含量、对 21 种抗生素的药敏性和生物膜形成进行了表征。通过 PFGE 确定了菌株的遗传相关性。此外，还研究了 lukS-PV 和 lukF-PV 的完整基因序列。所有菌株均对测试的抗生素敏感，且均为 mecA 阴性。三株菌株表现出相同的 PFGE 带型、ST152 克隆型和 t355 spa 型。在毒力因子基因检测中，未检测到 sea、seb、sec、sed、see、seg、seh、sei、sej、sek、sel、sem、sen、seo、sep、seq、seu、eta、etb、set1、geh 和 tst 基因。所有菌株均表现出α-和β-溶血素基因（hla 和 hlb）、蛋白酶编码基因（sspA、sspB 和 aur）、lukE 和 lukD 白细胞毒素基因（lukED）的阳性结果。这些菌株被发现为非生物膜形成者。通过本研究，描述了菌株的毒力特性，这是首次从食品中报道 PVL 阳性 MSSA 菌株的报告之一。
