用于对带电生物分子进行分离和电泳操作的电场隔离器（EFI）。

Electric field isolator (EFI) for isolated and electrophoretic manipulation of charged biomolecules.

作者信息

Yun Jae Young, Mun Sang Jun, Lee Seung S, Nam Hong Gil

机构信息

Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.

出版信息

Lab Chip. 2007 Jul;7(7):916-9. doi: 10.1039/b618099j. Epub 2007 Apr 20.

DOI:10.1039/b618099j

PMID:17594012

Abstract

This paper describes a novel technology-an electric field isolator (EFI)-that can be used for achieving isolated and electrophoretic manipulation of charged biomolecules inside a selected microscopic location. The EFI is a ground ring-shaped electrode (RE) surrounding a centre electrode (CE), which is comprised of a functional unit. When the CE is powered, the ground RE can inhibit the electric field from spreading to the neighbouring functional units. Therefore, the electrophoretic movement of the charged molecules in an electric field, which is based on the principle similar to that of electrophoresis, can be isolated inside a selected location. The ground RE causing this phenomenon is referred to as the EFI. In this paper, we clearly show the functionality of the EFI with mathematical and experimental studies.

摘要

本文描述了一种新型技术——电场隔离器（EFI），它可用于在选定的微观位置实现对带电生物分子的隔离和电泳操作。EFI是一个围绕中心电极（CE）的接地环形电极（RE），中心电极由一个功能单元组成。当中心电极通电时，接地环形电极可抑制电场扩散到相邻的功能单元。因此，基于与电泳原理相似的电场中带电分子的电泳运动可在选定位置内被隔离。导致这种现象的接地环形电极被称为EFI。在本文中，我们通过数学和实验研究清楚地展示了EFI的功能。