Slater G W, Noolandi J
Xerox Research Centre of Canada, Mississauga, Ontario.
Electrophoresis. 1988 Oct;9(10):643-6. doi: 10.1002/elps.1150091003.
A mathematical study of the effect of non-uniform electric fields on the width of DNA electrophoretic bands is presented. Using a simple model, we show that field gradients sharpen these bands during an experiment if the corresponding gradient of electrophoretic velocity is large enough. This is in agreement with experimental results indicating that narrower bands form when pulsed field electrophoresis is carried out in the presence of field gradients. Moreover, it is shown that there is in fact an optimal experimental duration that maximizes separation. Finally, gradients are also predicted to reduce the relative mobilities of the DNA fragments, which is a serious drawback of this technique.
本文对非均匀电场对DNA电泳条带宽度的影响进行了数学研究。通过一个简单的模型，我们表明，如果电泳速度的相应梯度足够大，在实验过程中场梯度会使这些条带变窄。这与实验结果一致，实验结果表明，在场梯度存在的情况下进行脉冲场电泳时会形成更窄的条带。此外，研究表明实际上存在一个使分离最大化的最佳实验持续时间。最后，预计场梯度也会降低DNA片段的相对迁移率，这是该技术的一个严重缺点。
