Cebrian Emma, Uriz María Jesús
Centre d'Estudis Avançats de Blanes, CSIC, C/ Accés a la Cala, St. Francesc s/n, 17300 Blanes, Girona, Spain.
Arch Environ Contam Toxicol. 2007 Nov;53(4):552-8. doi: 10.1007/s00244-006-0257-2. Epub 2007 Jun 26.
Mediterranean coastal areas are highly contaminated by heavy metals, which have been reported to produce harmful effects in marine organisms. Sponges are particularly vulnerable to waterborne metals because they are able to process large amounts of water. Dissociated sponge cells can move in response to external stimuli, and the cell body changes shape through production of pseudopodia and phylopodia. We studied for first time the effects of heavy metals (cadmium copper and mercury) on motility and aggregation of isolated sponge cells. Cell shape was assessed by using several shape indices. The three metals studied induced changes of different sign on cell shape. Mercury arrested movement of sponge cells, which tended to be rounded, without pseudopodia. In contrast, moderate concentrations of copper and cadmium enhanced pseudopodia formation and cell motility. On the other hand, the three metals enhanced cell aggregation at the concentrations assayed. Our results show that sponge cells respond to metal pollution in different ways and that these responses can be assessed by calculating several shape indices.
地中海沿岸地区受到重金属的高度污染，据报道，这些重金属会对海洋生物产生有害影响。海绵对水中的金属尤为敏感，因为它们能够处理大量的水。解离的海绵细胞可以响应外部刺激而移动，并且细胞体会通过产生伪足和叶状伪足而改变形状。我们首次研究了重金属（镉、铜和汞）对分离的海绵细胞运动性和聚集性的影响。通过使用几种形状指数来评估细胞形状。所研究的三种金属对细胞形状产生了不同符号的变化。汞阻止了海绵细胞的运动，细胞趋于圆形，没有伪足。相比之下，适度浓度的铜和镉增强了伪足的形成和细胞运动性。另一方面，在测定的浓度下，这三种金属都增强了细胞聚集。我们的结果表明，海绵细胞对金属污染有不同的反应方式，并且这些反应可以通过计算几种形状指数来评估。
