Bender Balázs, Bodrogi Lilla, Mayer Balázs, Schneider Zita, Zhao Yaofeng, Hammarström Lennart, Eggen André, Kacskovics Imre, Bosze Zsuzsanna
Agricultural Biotechnology Center, 2100, Szent-Györgyi A. st.4, Godollo, Hungary.
Transgenic Res. 2007 Oct;16(5):613-27. doi: 10.1007/s11248-007-9108-9. Epub 2007 Jun 27.
We generated and characterized transgenic mice carrying a 102 kb bovine genomic fragment, encoding the neonatal Fc receptor alpha-chain (bFcRn). FcRn plays a crucial role in the maternal IgG transport and it also regulates the IgG and albumin homeostasis. Some of its functions and transcriptional regulation show species specific differences. The FcRn heterodimer is composed of the alpha-chain and beta-2-microglobulin (beta2 m). A bacterial artificial chromosome containing the bovine FcRn alpha-chain gene (bFCGRT) with its 44 kb 5' and 50 kb long 3' flanking sequences was microinjected into fertilized mouse oocytes. Two of the transgenic lines generated, showed copy number related and integration site independent bFcRn expression. The bFcRn alpha-chain forms a functional receptor with the mouse beta2-microglobulin and extends the half-life of the mouse IgG in transgenic mice. Our results underline the feasibility of creating BAC transgenic mouse models of economically important bovine genes.
我们构建并鉴定了携带102 kb牛基因组片段的转基因小鼠，该片段编码新生Fc受体α链（bFcRn）。FcRn在母体IgG转运中起关键作用，还调节IgG和白蛋白的体内平衡。其一些功能和转录调控存在物种特异性差异。FcRn异二聚体由α链和β2微球蛋白（β2m）组成。将含有牛FcRnα链基因（bFCGRT）及其44 kb 5'和50 kb长3'侧翼序列的细菌人工染色体显微注射到受精的小鼠卵母细胞中。所产生的两个转基因品系显示出与拷贝数相关且与整合位点无关的bFcRn表达。bFcRnα链与小鼠β2微球蛋白形成功能性受体，并延长了转基因小鼠体内小鼠IgG的半衰期。我们的结果强调了创建具有经济重要性的牛基因的BAC转基因小鼠模型的可行性。
