Louveau I, Bonneau M, Gower D B
Station de Recherches Porcines, INRA, Saint Gilles, l'Hermitage, France.
Acta Endocrinol (Copenh). 1991 Nov;125(5):526-31. doi: 10.1530/acta.0.1250526.
The relationships between testicular 16-androstene and testosterone formation in young pigs and levels of 5 alpha-androstenone in fat exhibited at older ages were investigated. Testis tissue samples were taken from Large White male pigs at 75, 100 and 125 days of age (8 pigs per age group) and incubated with a mixture of [3H]progesterone and [14C]pregnenolone. 5 alpha-androstenone levels in fat were measured in all 24 pigs at 125, 145 and 170 days of age. Less androstadienone, more 3 alpha-androstenol and 11-fold more 3 beta-androstenol + androstadienol were synthesized from pregnenolone than from progesterone. 5 alpha-androstenone synthesis increased significantly between 100 and 125 days, whereas that of androstadienone declined between 75 and 100 days of age. Litter had a significant effect on all 16-androstene rates of formation. Fat 5 alpha-androstenone levels were positively correlated with 5 alpha-androstenone formation from both precursors. They were correlated with 3 beta-androstenol + androstadienol (positively) and testosterone (negatively) rates of synthesis from progesterone. It is concluded that in vitro measurements of 5 alpha-androstenone formation by testis tissue could be used as an early predictor of fat 5 alpha-androstenone levels exhibited at older ages.
研究了幼猪睾丸中16-雄烯生成与睾酮生成之间的关系，以及老龄猪脂肪中5α-雄烯酮水平。从75日龄、100日龄和125日龄的大白公猪采集睾丸组织样本（每个年龄组8头猪），并与[3H]孕酮和[14C]孕烯醇酮混合物一起孵育。在125日龄、145日龄和170日龄时测量了所有24头猪脂肪中的5α-雄烯酮水平。与从孕酮合成相比，从孕烯醇酮合成的雄二烯酮更少，3α-雄烯醇更多，3β-雄烯醇+雄二烯醇多11倍。5α-雄烯酮合成在100至125日龄之间显著增加，而雄二烯酮合成在75至100日龄之间下降。窝仔数对所有16-雄烯生成率有显著影响。脂肪中5α-雄烯酮水平与两种前体生成5α-雄烯酮均呈正相关。它们与3β-雄烯醇+雄二烯醇（正相关）和孕酮合成睾酮（负相关）的速率相关。得出的结论是，睾丸组织体外测量5α-雄烯酮生成可作为老龄猪脂肪中5α-雄烯酮水平的早期预测指标。
