Babol J, Squires E J, Lundström K
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala.
J Anim Sci. 1999 Jan;77(1):84-92. doi: 10.2527/1999.77184x.
The relationship between the metabolism of androsterone and skatole, the major compounds responsible for boar taint, was investigated in F4 Swedish Yorkshire x European Wild Pig intact males. The metabolism of androstenone and skatole were studied in liver microsomes, and the testicular steroid production was measured in testes microsomes. Including androstenone in the assays of skatole metabolism reduced the formation of 6-hydroxyskatole (pro-MII), and three other skatole metabolites (P<.05). The formation of three additional metabolites was not affected. Liver microsomal incubations of androstenone produced two metabolites, I and II. The rate of the formation of metabolite I and the rate of androstenone metabolism were correlated with the rate of skatole metabolism. Liver metabolism of androstenone was not related to levels of androstenone in fat. Testicular synthesis of 16-androstene steroids was correlated with combined synthesis of estrogens and androgens, plasma levels of androstenone, levels of skatole in fat, and skatole metabolism in the liver (P<.05). Plasma levels of estrone sulfate were correlated with levels of skatole in fat and with androstenone levels in fat and plasma and were negatively correlated with synthesis of skatole metabolite F-1 and pro-MII sulfation. These results indicate that the liver metabolism of androstenone and skatole are related. However, it is likely that the relationship between levels of androstenone and skatole in fat is due more to a link between the testicular synthesis of androstenone rather than to the metabolism of androstenone and skatole in the liver. Sex steroids may affect this relationship because of their biosynthesis along with androstenone and possible inhibition of skatole metabolism in the liver.
在F4代瑞典约克夏猪与欧洲野猪的杂交阉割公猪中，研究了雄烯酮和粪臭素（导致公猪膻味的主要化合物）的代谢之间的关系。在肝微粒体中研究了雄烯酮和粪臭素的代谢，并在睾丸微粒体中测定了睾丸类固醇的生成。在粪臭素代谢试验中加入雄烯酮可减少6-羟基粪臭素（前MII）及其他三种粪臭素代谢物的形成（P<0.05）。另外三种代谢物的形成未受影响。肝微粒体中雄烯酮的孵育产生了两种代谢物，I和II。代谢物I的形成速率和雄烯酮的代谢速率与粪臭素的代谢速率相关。雄烯酮的肝脏代谢与脂肪中雄烯酮的水平无关。睾丸中16-雄烯类固醇的合成与雌激素和雄激素的联合合成、血浆中雄烯酮的水平、脂肪中粪臭素的水平以及肝脏中粪臭素的代谢相关（P<0.05）。硫酸雌酮的血浆水平与脂肪中粪臭素的水平、脂肪和血浆中雄烯酮的水平相关，并且与粪臭素代谢物F-1的合成和前MII硫酸化呈负相关。这些结果表明，雄烯酮和粪臭素的肝脏代谢是相关的。然而，脂肪中雄烯酮和粪臭素水平之间的关系可能更多地归因于睾丸中雄烯酮合成之间的联系，而不是肝脏中雄烯酮和粪臭素的代谢。性类固醇可能会影响这种关系，因为它们与雄烯酮一起生物合成，并可能抑制肝脏中粪臭素的代谢。
