Sakagami M, Fukazawa K, Kitamura K, Doi K, Mori N, Matsunaga T
Department of Otolaryngology, Osaka University Medical School, Japan.
Acta Otolaryngol. 1991;111(5):872-8. doi: 10.3109/00016489109138424.
Unilateral endolymphatic hydrops was produced in guinea pigs by cauterization of the endolymphatic sac. Measurements of compound action potential (CAP), cochlear microphonics (CM) and negative summating potential (-SP) confirmed endolymphatic hydrops three months after surgery. In both control and hydropic ears, reaction product of HRP was observed only on the perilymphatic surface of the epithelial cells of Reissner's membrane after 10 min perfusion, while it was observed on both the endolymphatic and perilymphatic surfaces after 30 min perfusion. Epithelial tight junctions were not stained and labelled pinocytotic vesicles were observed in the epithelial cells. These findings suggest that the transport of HRP through Reissner's membrane is unchanged in endolymphatic hydrops and that the epithelial junctions are tight regardless of the distension of Reissner's membrane.
通过烧灼内淋巴囊在豚鼠中产生单侧内淋巴积水。术后三个月对复合动作电位（CAP）、耳蜗微音电位（CM）和负和电位（-SP）的测量证实了内淋巴积水。在对照耳和积水耳中，灌注10分钟后，仅在Reissner膜上皮细胞的外淋巴表面观察到辣根过氧化物酶（HRP）的反应产物，而灌注30分钟后，在内淋巴和外淋巴表面均观察到该反应产物。上皮紧密连接未被染色，且在上皮细胞中观察到标记的吞饮小泡。这些发现表明，在内淋巴积水中，HRP通过Reissner膜的转运未发生改变，并且无论Reissner膜是否扩张，上皮连接都是紧密的。
