Barham S S, Berlin J D, Brackeen R B
Cell Tissue Res. 1976 Feb 27;166(4):497-510. doi: 10.1007/BF00225914.
Electron microscopic cytochemistry was used to determine the localization of five phosphatase enzymes-glucose-6-phosphatase, inosine diphosphatase, thiamine pyrophosphatase, acid phosphatase, and adenosine triphosphatase-in control human testes. Glucose-6-phosphatase occurred in the endoplasmic reticulum and nuclear envelope of Sertoli cells, Leydig cells and primitive spermatogonia, but was not observed in more advanced spermatogenic cells. The presence of glucose-6-phosphatase activity paralleled the presence of glycogen in spermatogenic cells, i.e., both occurred in type AL and AD spermatogonia but not in type AP or B spermatogonia or in more advanced spermatogenic cells. Inosine diphosphatase activity was found in the endoplasmic reticulum, nuclear envelope, and Golgi complex of Sertoli cells and all spermatogenic cells except late spermatids. Additionally, inosine diphosphatase activity was localized at the junctions between Sertoli cells and late spermatids, but was not associated with any other plasma membrane. Thiamine pyrophosphatase reaction product was found in the Golgi bodies of Sertoli cells and in spermatogenic cells through immature spermatids. Neither inosine diphosphatase nor thiamine pyrophosphatase was observed in the Golgi bodies of spermatids during acrosomal formation. Acid phosphatase activity was found in lysosomes of spermatogonia, spermatocytes, and spermatids, in lysosomes of Leydig cells, and in lysosomes, lipofuscin bodies, and Golgi cisternae of Sertoli cells. It is thought that Sertoli lysosomes play a role in the phagocytosis of degenerating germ cells; however, the role of spermatogenic or Leydig lysosomes is unknown. Adenosine triphosphatase activity occurred at the interfaces between two spermatogonia, and between Sertoli cells and spermatogonia, but was not observed in the spaces between two Sertoli cells, two spermatocytes, two spermatids, or between Sertoli cells and spermatocytes, or between Sertoli cells and spermatids.
采用电子显微镜细胞化学方法，确定了对照人睾丸中5种磷酸酶——葡萄糖-6-磷酸酶、肌苷二磷酸酶、硫胺素焦磷酸酶、酸性磷酸酶和三磷酸腺苷酶的定位。葡萄糖-6-磷酸酶存在于支持细胞、间质细胞和原始精原细胞的内质网和核膜中，但在更高级别的生精细胞中未观察到。葡萄糖-6-磷酸酶活性的存在与生精细胞中糖原的存在平行，即二者均存在于AL型和AD型精原细胞中，但不存在于AP型或B型精原细胞或更高级别的生精细胞中。肌苷二磷酸酶活性见于支持细胞的内质网、核膜和高尔基体，以及除晚期精子细胞外的所有生精细胞。此外，肌苷二磷酸酶活性定位于支持细胞与晚期精子细胞之间的连接处，但与任何其他质膜均无关联。硫胺素焦磷酸酶反应产物见于支持细胞的高尔基体以及直至未成熟精子细胞阶段的生精细胞中。在顶体形成过程中，未在精子细胞的高尔基体中观察到肌苷二磷酸酶和硫胺素焦磷酸酶。酸性磷酸酶活性见于精原细胞、精母细胞和精子细胞的溶酶体中、间质细胞的溶酶体中，以及支持细胞的溶酶体、脂褐质小体和高尔基池。据认为，支持细胞的溶酶体在吞噬退化的生殖细胞中起作用；然而，生精细胞或间质细胞溶酶体的作用尚不清楚。三磷酸腺苷酶活性见于两个精原细胞之间以及支持细胞与精原细胞之间的界面处，但在两个支持细胞之间、两个精母细胞之间、两个精子细胞之间，或支持细胞与精母细胞之间，或支持细胞与精子细胞之间的间隙中未观察到。
