Barlow S B, Triemer R E
J Histochem Cytochem. 1986 Aug;34(8):1021-7. doi: 10.1177/34.8.3016072.
The distribution of four enzymes within the endomembrane system of the protist Crypthecodinium cohnii has been determined using cytochemical localizations with lead as a capture agent. Nucleoside diphosphatase (NDPase) activity, using inosine diphosphate (IDP) and thiamine pyrophosphate (TPP) as substrates, was observed in the Golgi apparatus, with a gradient of increasing reaction product noted in some cells from the cis to trans cisternae. Tubules and vesicles associated with the trans cisternae also contained reaction product. The endoplasmic reticulum exhibited a high activity of glucose-6-phosphatase [with glucose-6-phosphate (G-6-P) as substrate]. Traces of reaction product were also observed in the cis-most and trans-most cisternae of the dictyosomes. Activity of acid phosphatase (AcPase) was observed in Golgi cisternae as well as in associated cytoplasmic vesicles. Heaviest deposition was localized in medial and trans dictyosome cisternae. The cytoplasmic system of flattened vesicles subtending the surface membranes in these cells did not exhibit reactivity with any of the substrates used. The distribution of these enzymes in this algal cell appears similar to that observed in animal cells and suggests that these enzymes may represent markers for algal cell endomembrane compartments.
利用铅作为捕获剂进行细胞化学定位，已确定了原生生物卵形隐甲藻内膜系统中四种酶的分布。以二磷酸肌苷（IDP）和硫胺焦磷酸（TPP）为底物时，在高尔基体中观察到核苷二磷酸酶（NDPase）活性，在一些细胞中，从顺面到反面的潴泡中反应产物呈递增梯度。与反面潴泡相关的小管和囊泡也含有反应产物。内质网表现出高活性的葡萄糖-6-磷酸酶[以葡萄糖-6-磷酸（G-6-P）为底物]。在高尔基体的最顺面和最反面潴泡中也观察到微量反应产物。在高尔基体潴泡以及相关的细胞质囊泡中观察到酸性磷酸酶（AcPase）活性。最重的沉积物位于高尔基体中间和反面潴泡中。这些细胞中位于表面膜下方的扁平囊泡的细胞质系统对所用的任何底物均无反应。这些酶在这种藻类细胞中的分布似乎与在动物细胞中观察到的相似，表明这些酶可能代表藻类细胞内膜区室的标志物。
