使用定量PCR和培养方法来表征细菌生物膜中的生态通量。
Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms.
作者信息
Dalwai F, Spratt D A, Pratten J
机构信息
Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom.
出版信息
J Clin Microbiol. 2007 Sep;45(9):3072-6. doi: 10.1128/JCM.01131-07. Epub 2007 Jun 27.
Abstract
An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.
摘要
采用传统培养技术、分离株的16S rRNA基因序列比较分析以及定量聚合酶链反应（QPCR）对与牙龈炎相关的龈上菌斑体外模型进行了表征。在模拟牙龈炎的条件下，内氏放线菌、普氏菌属和牙龈卟啉单胞菌数量增加。与QPCR获得的估计值相比，培养法对革兰氏阴性菌和总细菌数量的估计明显偏低。
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