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使用定量PCR和培养方法来表征细菌生物膜中的生态通量。

Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms.

作者信息

Dalwai F, Spratt D A, Pratten J

机构信息

Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, United Kingdom.

出版信息

J Clin Microbiol. 2007 Sep;45(9):3072-6. doi: 10.1128/JCM.01131-07. Epub 2007 Jun 27.

DOI:10.1128/JCM.01131-07
PMID:17596351
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2045240/
Abstract

An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.

摘要

采用传统培养技术、分离株的16S rRNA基因序列比较分析以及定量聚合酶链反应（QPCR）对与牙龈炎相关的龈上菌斑体外模型进行了表征。在模拟牙龈炎的条件下，内氏放线菌、普氏菌属和牙龈卟啉单胞菌数量增加。与QPCR获得的估计值相比，培养法对革兰氏阴性菌和总细菌数量的估计明显偏低。

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Modeling shifts in microbial populations associated with health or disease.对与健康或疾病相关的微生物种群变化进行建模。
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Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.评估用于临床样本中总细菌载量评估的通用探针和引物组：牙髓病抗菌治疗中的一般意义和实际应用
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