Ghosal Abhisek, Bhowmick Rudra, Nandy Ranjan Kumar, Ramamurthy T, Chatterjee Nabendu Sekhar
National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata 700 010, India.
J Clin Microbiol. 2007 Sep;45(9):3068-71. doi: 10.1128/JCM.00646-07. Epub 2007 Jun 27.
Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.
产肠毒素大肠杆菌(ETEC)的定居因子抗原(CFAs)已根据其不同的抗原性分为几组。我们在此描述一种基于聚合酶链反应(PCR)的方法来检测ETEC的常见CFAs,这些CFAs通过传统血清学进行了表征。该PCR检测方法对于检测已表达的CFA基因而言简单且灵敏。