基于聚合酶链反应的产肠毒素大肠杆菌常见定植因子抗原的鉴定
PCR-based identification of common colonization factor antigens of enterotoxigenic Escherichia coli.
作者信息
Ghosal Abhisek, Bhowmick Rudra, Nandy Ranjan Kumar, Ramamurthy T, Chatterjee Nabendu Sekhar
机构信息
National Institute of Cholera and Enteric Diseases, P33 CIT Road, Scheme XM, Beliaghata, Kolkata 700 010, India.
出版信息
J Clin Microbiol. 2007 Sep;45(9):3068-71. doi: 10.1128/JCM.00646-07. Epub 2007 Jun 27.
Abstract
Colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli (ETEC) have been classified into several groups based on their distinct antigenicity. We describe here a PCR-based method to detect common CFAs of ETEC, which were characterized using conventional serology. This PCR assay is simple and sensitive for the detection of expressed CFA genes.
摘要
产肠毒素大肠杆菌（ETEC）的定居因子抗原（CFAs）已根据其不同的抗原性分为几组。我们在此描述一种基于聚合酶链反应（PCR）的方法来检测ETEC的常见CFAs，这些CFAs通过传统血清学进行了表征。该PCR检测方法对于检测已表达的CFA基因而言简单且灵敏。
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