Claudino M J C, Soares D, Van Keulen F, Marques M P C, Cabral J M S, Fernandes P
Departamento de Engenharias e Ciências Naturais, Universidade Lusófona de Humanidades e Tecnologias, Av. Campo Grande 376, 1749-024 Lisboa, Portugal.
Bioresour Technol. 2008 May;99(7):2304-11. doi: 10.1016/j.biortech.2007.05.007. Epub 2007 Jun 26.
Silicone rubbers are hydrophobic, a feature that may prove advantageous if this material is to be used as immobilization matrix in bioconversion systems where hydrophobic species are present, such as sterols and mycobacterial cells. Mycobacterium sp. cells with sitosterol side chain cleavage activity were accordingly effectively adsorbed onto silicone and the potential application of the concept was assessed by matching the behavior of the resulting immobilized biocatalyst with free cells and Celite immobilized cells. Mass transfer, kinetics, thermal and storage stability characterization of a biotransformation system based in the use of the silicone immobilized biocatalyst was performed. The feasibility of biocatalyst reutilization was tentatively explored.
硅橡胶具有疏水性，如果将这种材料用作生物转化系统中的固定化基质，而该系统中存在疏水性物质，如甾醇和分枝杆菌细胞，那么这一特性可能会被证明是有利的。相应地，具有谷甾醇侧链裂解活性的分枝杆菌属细胞被有效地吸附到硅橡胶上，并通过将所得固定化生物催化剂与游离细胞和硅藻土固定化细胞的行为进行匹配，评估了该概念的潜在应用。对基于使用硅橡胶固定化生物催化剂的生物转化系统进行了传质、动力学、热稳定性和储存稳定性表征。初步探索了生物催化剂再利用的可行性。
