能够从甾醇产生9α-羟基雄烯二酮的分枝杆菌属突变菌株。
Mycobacterium sp. mutant strain producing 9alpha-hydroxyandrostenedione from sitosterol.
作者信息
Donova M V, Gulevskaya S A, Dovbnya D V, Puntus I F
机构信息
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Postbox 142290, Pushchino, Moscow Region, Russia.
出版信息
Appl Microbiol Biotechnol. 2005 Jun;67(5):671-8. doi: 10.1007/s00253-004-1808-y. Epub 2005 Jan 13.
Mycobacterium sp. VKM Ac-1815D and its derivatives with altered resistance to antibacterial agents were able to produce androst-4-ene-3,17-dione (AD) as a major product from sitosterol. In this study, those strains were subjected to subsequent mutagenization by chemical agents and UV irradiation in combination with sitosterol selection pressure. The mutant Mycobacterium sp. 2-4 M was selected, being capable of producing 9alpha-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) as a major product from sitosterol, with a 50% molar yield. Along with 9-OH-AD, both AD and 9alpha-hydroxylated metabolites with a partially degraded side-chain were formed from sitosterol by the mutant strain. The strain was unable to degrade 9-OH-AD, but degraded androsta-1,4-diene-3,17-dione (ADD), thus indicating a deficiency in steroid 1(2)-dehydrogenase and the presence of 9alpha-hydroxylase activity.
分枝杆菌属VKM Ac-1815D及其对抗菌剂耐药性改变的衍生物能够将谷甾醇作为主要底物生成雄甾-4-烯-3,17-二酮（AD）。在本研究中，这些菌株在谷甾醇选择压力下，通过化学试剂诱变和紫外线照射进行后续诱变处理。筛选出了突变型分枝杆菌属2-4 M，它能够将谷甾醇作为主要底物生成9α-羟基雄甾-4-烯-3,17-二酮（9-OH-AD），摩尔产率为50%。该突变菌株利用谷甾醇生成9-OH-AD的同时，还生成了AD以及侧链部分降解的9α-羟基化代谢产物。该菌株无法降解9-OH-AD，但能够降解雄甾-1,4-二烯-3,17-二酮（ADD），这表明该菌株缺乏甾体1(2)-脱氢酶，而具有9α-羟化酶活性。
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