Pielage Julia F, Cichon Christoph, Greune Lilo, Hirashima Mitsuomi, Kucharzik Torsten, Schmidt M Alexander
Institute of Infectiology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, von-Esmarch-Str. 56, 48149 Münster, Germany.
Int J Biochem Cell Biol. 2007;39(10):1886-901. doi: 10.1016/j.biocel.2007.05.009. Epub 2007 May 24.
M cells interspersed in the follicle-associated epithelium of Peyer's patches represent the major antigen sampling cells of the intestinal mucosa providing immune surveillance for particulate antigens. Despite their crucial role in immune defense our knowledge about these elusive cells is still only rudimentary. A Caco-2 co-culture model for the induction of M cell-like cells and DNA microarray analysis for differential gene expression profiling were employed to identify (a) putative suitable surface marker(s). Induction of M cell-like cells was demonstrated morphologically by electron microscopy, evaluated by infection with Yersinia enterocolitica and enteropathogenic Escherichia coli strain E2348/69 and further monitored by changes in binding of the lectin UEA-1. The differentiation of Caco-2 cells was found to be reversible, dependent on (a) lymphocyte-derived soluble factor(s) and accompanied by the up-regulation of the glycoprotein lectin galectin-9, which was specifically expressed on these cells as well as on human follicle-associated epithelial (FAE) cells. Galectin-9 represents a novel surface marker which might be employed for molecular targeting to the Peyer's patches thereby opening new opportunities for drug and vaccine development.
散布于派尔集合淋巴结滤泡相关上皮中的M细胞是肠黏膜主要的抗原采样细胞，负责对颗粒性抗原进行免疫监测。尽管它们在免疫防御中发挥着关键作用，但我们对这些难以捉摸的细胞的了解仍然十分有限。我们采用一种用于诱导M细胞样细胞的Caco-2共培养模型以及用于差异基因表达谱分析的DNA微阵列分析来鉴定（一种或多种）假定合适的表面标志物。通过电子显微镜从形态学上证实了M细胞样细胞的诱导，通过小肠结肠炎耶尔森菌和肠致病性大肠杆菌菌株E2348/69感染进行评估，并通过凝集素UEA-1结合的变化进一步监测。发现Caco-2细胞的分化是可逆的，依赖于一种或多种淋巴细胞衍生的可溶性因子，并伴随着糖蛋白凝集素半乳糖凝集素-9的上调，该凝集素在这些细胞以及人滤泡相关上皮（FAE）细胞上特异性表达。半乳糖凝集素-9代表一种新的表面标志物，可用于分子靶向派尔集合淋巴结，从而为药物和疫苗开发开辟新的机会。
