加味四君子汤对小鼠速发型过敏反应的影响。

Effect of Gamiseunggal-Tang on immediate type allergic reaction in mice.

作者信息

Jeong Hyun-Ja, Moon Phil-Dong, Um Jae-Young, Park Jinhan, Leem Kang-Hyun, Kim Chang-Ju, Kim Hyung-Min, Hong Seung-Heon

机构信息

Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, Seoul, South Korea.

出版信息

Indian J Med Res. 2007 Apr;125(4):542-9.

PMID:17598940

Abstract

BACKGROUND & OBJECTIVE: The herbal formulation, Gamiseunggal-Tang (G-Tang) has long been used for various allergic diseases. The mechanism of its action is largely unknown. We carried out this study to determine the effect of G-Tang on the mast cell-mediated anaphylactic reactions in vivo and in vitro murine models.

METHODS

In this study, the effects of G-Tang on the mast cell-mediated anaphylactic reactions were examined by using the ear swelling, histamine assay, and ELISA method in murine model.

RESULTS

Anal administration of G-Tang showed dose-dependent inhibitory activity on the compound 48/80-induced ear swelling response (P<0.05) and histamine release (P<0.01). G-Tang (0.001-0.1 g/kg) significantly inhibited passive cutaneous anaphylaxis (P<0.05) in mice. The production of tumour necrosis factor-alpha (TNF-alpha) was also significantly inhibited (about 47.4%, at 0.1 mg/ml, P<0.01) by treatment of G-tang in anti-dinitrophenyl IgE antibodystimulated mast cells.

INTERPRETATION & CONCLUSION: Findings of our study showed that G-Tang inhibited immediate type allergic reaction in a murine model and may be beneficial in the treatment of allergic inflammatory diseases.

摘要

背景与目的

草药配方加味升麻汤（G-Tang）长期以来用于多种过敏性疾病。其作用机制尚不清楚。我们开展本研究以确定加味升麻汤在体内和体外小鼠模型中对肥大细胞介导的过敏反应的影响。

方法

在本研究中，通过小鼠模型中的耳部肿胀、组胺测定和酶联免疫吸附测定（ELISA）方法检测加味升麻汤对肥大细胞介导的过敏反应的影响。

结果

腹腔注射加味升麻汤对化合物48/80诱导的耳部肿胀反应（P<0.05）和组胺释放（P<0.01）显示出剂量依赖性抑制活性。加味升麻汤（0.001 - 0.1 g/kg）显著抑制小鼠被动皮肤过敏反应（P<0.05）。在抗二硝基苯基IgE抗体刺激的肥大细胞中，加味升麻汤处理也显著抑制肿瘤坏死因子-α（TNF-α）的产生（在0.1 mg/ml时约为47.4%，P<0.01）。