Urata Hidehito, Nomura Kanba, Wada Shun-ichi, Akagi Masao
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Biochem Biophys Res Commun. 2007 Aug 24;360(2):459-63. doi: 10.1016/j.bbrc.2007.06.075. Epub 2007 Jun 19.
One of the intriguing applications of aptamers is sensing molecules. In principle, an aptamer can specifically recognize and bind to a unique ligand, leading to a structural change of an aptamer. By acquiring information for the structural change, the detection of the ligand can be achieved. To design and explore an aptamer molecule to detect adenosine, we have synthesized some ATP aptamer variants labeled with donor and acceptor fluorophores. Although the fluorescent response of the aptamer variants was highly dependent on experimental temperature, we have found one of the variants showing suitable fluorescent response by titration with adenosine. The aptamer variant showed remarkable selectivity for adenosine over the other ribonucleosides. On the other hand, the enantio-specificity of the aptamer variant in the ligand recognition was not enough to selectively detect d-adenosine over l-adenosine.
适体的有趣应用之一是传感分子。原则上，适体可以特异性识别并结合独特的配体，导致适体的结构发生变化。通过获取结构变化的信息，就可以实现对配体的检测。为了设计和探索用于检测腺苷的适体分子，我们合成了一些标记有供体和受体荧光团的ATP适体变体。尽管适体变体的荧光响应高度依赖于实验温度，但我们通过用腺苷滴定发现了其中一种变体表现出合适的荧光响应。该适体变体对腺苷显示出比其他核糖核苷更高的选择性。另一方面，该适体变体在配体识别中的对映体特异性不足以选择性地检测d-腺苷而不是l-腺苷。
