Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
Sensors (Basel). 2010;10(2):1355-76. doi: 10.3390/s100201355. Epub 2010 Feb 12.
Fluorescent biosensors to detect the bona fide events of biologically important molecules in living cells are increasingly demanded in the field of molecular cell biology. Recent advances in the development of fluorescent biosensors have made an outstanding contribution to elucidating not only the roles of individual biomolecules, but also the dynamic intracellular relationships between these molecules. However, rational design strategies of fluorescent biosensors are not as mature as they look. An insatiable request for the establishment of a more universal and versatile strategy continues to provide an attractive alternative, so-called modular strategy, which permits facile preparation of biosensors with tailored characteristics by a simple combination of a receptor and a signal transducer. This review describes an overview of the progress in design strategies of fluorescent biosensors, such as auto-fluorescent protein-based biosensors, protein-based biosensors covalently modified with synthetic fluorophores, and signaling aptamers, and highlights the insight into how a given receptor is converted to a fluorescent biosensor. Furthermore, we will demonstrate a significance of the modular strategy for the sensor design.
荧光生物传感器越来越多地被用于检测活细胞中生物重要分子的真实事件，这在分子细胞生物学领域受到了越来越多的关注。荧光生物传感器的发展的最新进展不仅对阐明单个生物分子的作用，而且对阐明这些分子之间的动态细胞内关系做出了杰出的贡献。然而，荧光生物传感器的合理设计策略并不像看起来那么成熟。人们对建立更通用和多功能策略的需求不断增加，因此出现了一种有吸引力的替代方法，即所谓的模块化策略，通过受体和信号转导器的简单组合，可以方便地制备具有定制特性的生物传感器。本文综述了荧光生物传感器设计策略的进展，例如基于自发荧光蛋白的生物传感器、用合成荧光团共价修饰的蛋白基生物传感器和信号适体，并重点介绍了如何将给定的受体转化为荧光生物传感器的见解。此外，我们将展示模块化策略在传感器设计中的重要性。
