Steven F S
Connect Tissue Res. 1975;4(1):1-6. doi: 10.3109/03008207509152190.
The enzymic solubilisation of insoluble collagen fibrils by bacterial collagenase has been examined employing unlabelled polymeric collagen fibrils (PC), rhodamine labelled fibrils (RB-PC), and fluorescein labelled fibrils (F-PC) as substrates. The presence of the label did not affect the kinetics of enzyme digestion but enabled the solubilized products to be rapidly estimated. The quantity of substrate used was found to play a major role in determining the quantity of peptides solubilised by a given enzyme concentration. It is suggested that in the PC fibrils only a limited number of TC molecules were suitably located to form an enzyme substrate complex with bacterial collagenase. Conditions were found in which the F-PC and RB-PC could be used as substrates in the assay of this enzyme.
利用未标记的聚合胶原纤维（PC）、罗丹明标记的纤维（RB-PC）和荧光素标记的纤维（F-PC）作为底物，研究了细菌胶原酶对不溶性胶原纤维的酶促溶解作用。标记物的存在不影响酶消化的动力学，但能快速估算溶解产物的量。发现所用底物的量在决定给定酶浓度下溶解的肽量方面起主要作用。有人提出，在PC纤维中，只有有限数量的TC分子处于合适的位置，以与细菌胶原酶形成酶-底物复合物。已找到一些条件，在此条件下F-PC和RB-PC可作为该酶测定的底物。
