Williams John, Wood JodiAnne, Pandarinathan Lakshmipathi, Karanian David A, Bahr Ben A, Vouros Paul, Makriyannis Alexandros
Center for Drug Discovery, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts 02115, USA.
Anal Chem. 2007 Aug 1;79(15):5582-93. doi: 10.1021/ac0624086. Epub 2007 Jun 29.
The endocannabinoid system's biological significance continues to grow as novel endocannabinoid metabolites are discovered. Accordingly, a myopic view of the system that focuses solely on one or two endocannabinoids, such as anandamide or 2-arachidonoyl glycerol, is insufficient to describe the biological responses to perturbations of the system. Rather, the endocannabinoid metabolome as a whole must be analyzed. The work described here is based on liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometry. This method has been validated to quantify, in a single chromatographic run, the levels of 15 known or suspected metabolites of the endocannabinoid system in the rat brain and is applicable to other biological matrixes. We have obtained an endocannabinoid profile specifically for the frontal cortex of the rat brain and have determined anandamide level differences following the administration of the fatty acid amide hydrolase inhibitor AM374.
随着新型内源性大麻素代谢物的发现，内源性大麻素系统的生物学意义不断凸显。因此，仅关注一种或两种内源性大麻素（如花生四烯乙醇胺或2-花生四烯酸甘油酯）的该系统的近视观点，不足以描述对该系统扰动的生物学反应。相反，必须对整个内源性大麻素代谢组进行分析。此处描述的工作基于液相色谱与大气压化学电离质谱联用。该方法已得到验证，可在一次色谱运行中定量大鼠脑中内源性大麻素系统的15种已知或疑似代谢物的水平，并且适用于其他生物基质。我们已经获得了大鼠脑额叶皮质特有的内源性大麻素谱，并确定了在给予脂肪酸酰胺水解酶抑制剂AM374后花生四烯乙醇胺水平差异。
