Aarestrup Frank M, Knöchel Susanne, Hasman Henrik
National Food Institute, Technical University of Denmark, Copenhagen, Denmark.
Foodborne Pathog Dis. 2007 Summer;4(2):216-21. doi: 10.1089/fpd.2006.0078.
This study was conducted to determine the susceptibility of Listeria monocytogenes isolated from food products to antimicrobial agents commonly used for treatment of infections with gram-positive bacteria, and to disinfectants. A total of 114 L. monocytogenes retail isolates were tested for susceptibility to ceftiofur, chloramphenicol, ciprofloxacin, erythromycin, florfenicol, penicillin, spectinomycin, streptomycin, tetracycline, tiamulin, trimethoprim, and co-trimoxazole, and the disinfectants benzalkonium chloride and triclosan, by determination of minimum inhibitory concentrations (MICs). All isolates were resistant to ceftiofur, but susceptible to the other antibiotics. A single isolate had a MIC of 4 mg/L for ciprofloxacin. For tiamulin. the MIC values were around the breakpoint used. Most isolates had MICs for triclosan at 16 mg/L. The MICs for benzalkonium chloride formed a bimodal distribution, with 105 isolates having a MIC of 4 mg/L and 9 isolates MICs of 16 and 32 mg/L. This study showed that Danish isolates of L. monocytogenes have not developed or acquired resistance to antimicrobial agents used for treatment or disinfection, except for benzalkonium chloride. The MICs for triclosan was high compared to other gram-positive bacteria, suggesting that triclosan might not be useful against L. monocytogenes if incorporated in materials in the food industry.
本研究旨在确定从食品中分离出的单核细胞增生李斯特菌对常用于治疗革兰氏阳性菌感染的抗菌剂以及消毒剂的敏感性。通过测定最低抑菌浓度（MIC），对总共114株零售单核细胞增生李斯特菌分离株进行了对头孢噻呋、氯霉素、环丙沙星、红霉素、氟苯尼考、青霉素、壮观霉素、链霉素、四环素、替米考星、甲氧苄啶和复方新诺明，以及消毒剂苯扎氯铵和三氯生的敏感性测试。所有分离株对头孢噻呋耐药，但对其他抗生素敏感。有一株分离株对环丙沙星的MIC为4mg/L。对于替米考星，MIC值接近所用的断点值。大多数分离株对三氯生的MIC为16mg/L。苯扎氯铵的MIC呈双峰分布，105株分离株的MIC为4mg/L，9株分离株的MIC为16mg/L和32mg/L。本研究表明，丹麦单核细胞增生李斯特菌分离株除对苯扎氯铵外，尚未对用于治疗或消毒的抗菌剂产生或获得耐药性。与其他革兰氏阳性菌相比，三氯生的MIC较高，这表明如果将三氯生添加到食品工业材料中，可能对单核细胞增生李斯特菌无效。
