Jia Yingmin, Nightingale Kendra K, Boor Kathryn J, Ho Alphina, Wiedmann Martin, McGann Patrick
College of Food Science and Technology, Agricultural University of Hebei, Baoding, China.
Foodborne Pathog Dis. 2007 Summer;4(2):222-32. doi: 10.1089/fpd.2006.0081.
Listeria monocytogenes is a human foodborne pathogen with a broad range of hosts. While the L. monocytogenes genome encodes a number of internalins, which are leucine-rich repeat bacterial surface proteins with putative or confirmed roles in host cell attachment and invasion, the specific function of many internalins remains unclear. The distribution of 7 internalin genes (inlC, inlC2, inlD, inlE, inlF, inlG, and inlH) in 120 L. monocytogenes isolates from humans, foods, and animals was investigated to determine if the distribution of these proteins differed with respect to source or phylogenetic lineage. Isolates were classified into 6 different profiles based on internalin gene presence or absence, and a phylogeny based on one stress response (sigB) and two housekeeping (gap and prs) genes was used to correlate these profiles with L. monocytogenes phylogenetic lineages. All 69 isolates classified into L. monocytogenes lineage I, which is overrepresented among human disease cases, had the same internalin profile (presence of inlC and the inlC2DE operon). Lineage II (48 isolates), which is common among food and environmental sources, represented 4 internalin gene profiles, with most isolates carrying inlG and inlF in addition to inlC and inlC2DE. Our data indicate that L. monocytogenes isolates show diverse and distinct patterns of internalin gene presence/absence and L. monocytogenes internalin profiles are associated with phylogenetic lineages.
单核细胞增生李斯特菌是一种可通过食物传播给人类的病原体，宿主范围广泛。虽然单核细胞增生李斯特菌的基因组编码了多种内化素，这些内化素是富含亮氨酸重复序列的细菌表面蛋白，在宿主细胞附着和侵袭中具有假定或已证实的作用，但许多内化素的具体功能仍不清楚。研究了120株来自人类、食品和动物的单核细胞增生李斯特菌分离株中7种内化素基因（inlC、inlC2、inlD、inlE、inlF、inlG和inlH）的分布情况，以确定这些蛋白质的分布在来源或系统发育谱系方面是否存在差异。根据内化素基因的有无，将分离株分为6种不同的谱型，并基于一种应激反应基因（sigB）和两种管家基因（gap和prs）构建系统发育树，以将这些谱型与单核细胞增生李斯特菌的系统发育谱系相关联。所有69株被归类为单核细胞增生李斯特菌I系的分离株，在人类疾病病例中占比过高，具有相同的内化素谱型（存在inlC和inlC2DE操纵子）。II系（48株分离株）在食品和环境来源中很常见，代表4种内化素基因谱型，大多数分离株除了携带inlC和inlC2DE外，还携带inlG和inlF。我们的数据表明，单核细胞增生李斯特菌分离株显示出不同且独特的内化素基因存在/缺失模式，并且单核细胞增生李斯特菌的内化素谱型与系统发育谱系相关。
