Watanabe Karen H, Jensen Kathleen M, Orlando Edward F, Ankley Gerald T
Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health & Science University, 20000 N.W. Walker Rd., Beaverton, OR 97006, USA.
Comp Biochem Physiol C Toxicol Pharmacol. 2007 Sep;146(3):348-56. doi: 10.1016/j.cbpc.2007.04.015. Epub 2007 May 13.
Jensen et al. [Jensen, K.M., Korte, J.J., Kahl, M.D., Pasha, M.S., Ankley, G.T., 2001. Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas). Comp. Biochem. Physiol. C 128, 127-141.] investigated aspects of the normal reproductive biology of the fathead minnow (FHM, P. promelas), and subsequent studies have generated a large amount of additional reproductive data for endpoints such as plasma steroid hormone and vitellogenin concentrations, spawn interval, and secondary sex characteristics (i.e., nuptial tubercle score and fat pad weight). These data were analyzed and fitted with statistical distributions to improve understanding of the variability in normal, unexposed, adult male (n=154) and female (n=186) FHM. Summary statistics for most endpoints were consistent with results from other more limited studies of FHMs. Male fat pad weight, and in both sexes gonad and liver weights were found to be proportional to body weight. Multiple statistical distributions were found to characterize each endpoint with the exception of spawn interval. Based on one of the largest datasets ever compiled for controlled studies, results presented herein provide a robust point of reference for the quantitative assessment of reproductive processes in the fathead minnow.
詹森等人[詹森，K.M.，科尔特，J.J.，卡尔，M.D.，帕夏，M.S.，安克利，G.T.，2001年。黑头呆鱼（肥头鲦鱼，Pimephales promelas）的基础生殖生物学和内分泌学方面。比较生物化学与生理学C 128，127 - 141。]研究了黑头呆鱼（FHM，P. promelas）正常生殖生物学的各个方面，随后的研究针对血浆类固醇激素和卵黄蛋白原浓度、产卵间隔以及第二性征（即婚瘤评分和脂肪垫重量）等终点指标生成了大量额外的生殖数据。对这些数据进行了分析并拟合统计分布，以增进对正常、未接触过污染物的成年雄性（n = 154）和雌性（n = 186）FHM变异性的理解。大多数终点指标的汇总统计数据与其他对FHM进行的更有限研究结果一致。发现雄性脂肪垫重量以及两性的性腺和肝脏重量均与体重成正比。除产卵间隔外，发现多种统计分布可表征每个终点指标。基于有史以来为对照研究汇编的最大数据集之一，本文给出的结果为定量评估黑头呆鱼的生殖过程提供了一个有力的参考点。
