US Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Mid-Continent Ecology Division, 6201 Congdon Boulevard, Duluth, MN 55804, USA.
Ecotoxicol Environ Saf. 2010 May;73(4):472-7. doi: 10.1016/j.ecoenv.2009.09.007. Epub 2009 Sep 26.
Neurotransmitters such as dopamine play an important role in regulating fish reproduction. However, the potential for neuroendocrine active chemicals to disrupt fish reproduction has not been well studied, despite emerging evidence of their discharge into aquatic environments. This study is the first to apply the fathead minnow 21 d reproduction assay developed for the US Endocrine Disruptor Screening Program to evaluate the reproductive toxicity of a model neuroendocrine active chemical, the dopamine 2 receptor antagonist, haloperidol. Continuous exposure to up to 20 imcrog haloperidol/L had no significant effects on fathead minnow fecundity, secondary sex characteristics, gonad histology, or plasma steroid and vitellogenin concentrations. The only significant effect observed was an increase in gonadotropin-releasing hormone (cGnRH) transcripts in the male brain. Results suggest that non-lethal concentrations of haloperidol do not directly impair fish reproduction. Potential effects of haloperidol on reproductive behaviors and gene expression were examined in a companion study.
神经递质，如多巴胺，在调节鱼类生殖方面发挥着重要作用。然而，尽管有越来越多的证据表明神经内分泌活性化学物质会排放到水生环境中，但它们对鱼类生殖的潜在干扰作用还没有得到很好的研究。本研究首次应用美国内分泌干扰物筛选计划开发的黑头呆鱼 21 天繁殖试验，评估了一种模型神经内分泌活性化学物质，即多巴胺 2 受体拮抗剂氟哌啶醇的生殖毒性。连续暴露于高达 20 微克/升的氟哌啶醇对黑头呆鱼的繁殖力、次要性特征、性腺组织学或血浆类固醇和卵黄蛋白原浓度没有显著影响。唯一观察到的显著影响是雄性大脑中促性腺激素释放激素（cGnRH）转录本的增加。结果表明，非致死浓度的氟哌啶醇不会直接损害鱼类的生殖能力。在一项配套研究中，还研究了氟哌啶醇对生殖行为和基因表达的潜在影响。
