Nuhiji Edin, Mulvaney Paul
School of Chemistry, The University of Melbourne, Parkville, Victoria 3010, Australia.
Small. 2007 Aug;3(8):1408-14. doi: 10.1002/smll.200600676.
The development of an inexpensive, highly sensitive, whispering gallery mode (WGM) based biosensing system is demonstrated. The system comprises a silica microsphere functionalized with a fluorophore and a dense monolayer of single-strand oligonucleotides. The adsorption of the complementary strand causes spectral shifts in the emission spectrum of the microsphere that can be registered using a conventional optical microscope and CCD detector. The system is capable of detecting low-abundance, unlabeled-oligonucleotide targets. The spectral shifts can be used to monitor both the hybridization kinetics and the denaturation of the double-strand DNA at elevated temperatures. The hybridization appears to be completely reversible. Complementary oligonucleotide probes more than 30 bases in length are most readily detected while a complete assay takes only a few minutes.
展示了一种基于廉价、高灵敏度回音壁模式（WGM）的生物传感系统的开发。该系统包括一个用荧光团功能化的二氧化硅微球和一个单链寡核苷酸的致密单层。互补链的吸附会导致微球发射光谱的光谱位移，这可以使用传统光学显微镜和电荷耦合器件（CCD）探测器进行记录。该系统能够检测低丰度、未标记的寡核苷酸靶标。光谱位移可用于监测高温下双链DNA的杂交动力学和变性。杂交似乎是完全可逆的。长度超过30个碱基的互补寡核苷酸探针最容易被检测到，而完整的检测只需几分钟。
