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使用大规模平行签名测序（MPSS）发现人类肝细胞的多种转录本。

Discovering multiple transcripts of human hepatocytes using massively parallel signature sequencing (MPSS).

作者信息

Huang Jian, Hao Pei, Zhang Yun-Li, Deng Fu-Xing, Deng Qing, Hong Yi, Wang Xiao-Wo, Wang Yun, Li Ting-Ting, Zhang Xue-Gong, Li Yi-Xue, Yang Peng-Yuan, Wang Hong-Yang, Han Ze-Guang

机构信息

Department of Chemistry of Fudan University & Shanghai-Ministry Key Laboratory of Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, China.

出版信息

BMC Genomics. 2007 Jul 2;8:207. doi: 10.1186/1471-2164-8-207.

DOI:10.1186/1471-2164-8-207
PMID:17601345
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1929076/
Abstract

BACKGROUND

The liver is the largest human internal organ--it is composed of multiple cell types and plays a vital role in fulfilling the body's metabolic needs and maintaining homeostasis. Of these cell types the hepatocytes, which account for three-quarters of the liver's volume, perform its main functions. To discover the molecular basis of hepatocyte function, we employed Massively Parallel Signature Sequencing (MPSS) to determine the transcriptomic profile of adult human hepatocytes obtained by laser capture microdissection (LCM).

RESULTS

10,279 UniGene clusters, representing 7,475 known genes, were detected in human hepatocytes. In addition, 1,819 unique MPSS signatures matching the antisense strand of 1,605 non-redundant UniGene clusters (such as APOC1, APOC2, APOB and APOH) were highly expressed in hepatocytes.

CONCLUSION

Apart from a large number of protein-coding genes, some of the antisense transcripts expressed in hepatocytes could play important roles in transcriptional interference via a cis-/trans-regulation mechanism. Our result provided a comprehensively transcriptomic atlas of human hepatocytes using MPSS technique, which could be served as an available resource for an in-depth understanding of human liver biology and diseases.

摘要

背景

肝脏是人体最大的内部器官，由多种细胞类型组成，在满足身体代谢需求和维持体内平衡方面发挥着至关重要的作用。在这些细胞类型中，占肝脏体积四分之三的肝细胞执行其主要功能。为了发现肝细胞功能的分子基础，我们采用大规模平行签名测序（MPSS）来确定通过激光捕获显微切割（LCM）获得的成人肝细胞的转录组概况。

结果

在人类肝细胞中检测到10279个单基因簇，代表7475个已知基因。此外，1819个独特的MPSS签名与1605个非冗余单基因簇（如APOC1、APOC2、APOB和APOH）的反义链匹配，在肝细胞中高表达。

结论

除了大量的蛋白质编码基因外，肝细胞中表达的一些反义转录本可能通过顺式/反式调节机制在转录干扰中发挥重要作用。我们的结果利用MPSS技术提供了一份全面的人类肝细胞转录组图谱，可作为深入了解人类肝脏生物学和疾病的可用资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/571bcec4d0d2/1471-2164-8-207-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/511ec3e90869/1471-2164-8-207-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/0d4c21dadb34/1471-2164-8-207-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/abb46aa30142/1471-2164-8-207-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/d8abdd7113ae/1471-2164-8-207-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/f4304208b21c/1471-2164-8-207-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/540f1afb7bc7/1471-2164-8-207-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/571bcec4d0d2/1471-2164-8-207-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/511ec3e90869/1471-2164-8-207-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/0d4c21dadb34/1471-2164-8-207-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/abb46aa30142/1471-2164-8-207-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/d8abdd7113ae/1471-2164-8-207-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/f4304208b21c/1471-2164-8-207-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/540f1afb7bc7/1471-2164-8-207-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a19/1929076/571bcec4d0d2/1471-2164-8-207-7.jpg

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