Díaz P, Juárez A
Departament de Microbiología, Facultat de Biología Universitat de Barcelona, Spain.
Microbiologia. 1991 Sep;7(2):74-81.
A genomic library of Sau 3AI-generated Serratia marcescens DNA fragments was constructed in Escherichia coli 5K harbouring plasmid pANN202-312. Two clones capable of increasing the external hemolytic activity of the parental strain were isolated and characterized. The recombinant plasmids purified from these clones (designated pPSF29 and pPSH18) contained approximately 30 and 20 Kb, respectively, of Serratia DNA. Introduction of either plasmid in Escherichia coli caused a marked increase in hemolysin production and a concomitant decrease in cell viability.
利用Sau 3AI酶切产生的粘质沙雷氏菌DNA片段构建了基因组文库，该文库构建于携带质粒pANN202 - 312的大肠杆菌5K中。分离并鉴定了两个能够增强亲本菌株体外溶血活性的克隆。从这些克隆中纯化的重组质粒（分别命名为pPSF29和pPSH18）分别含有约30 kb和20 kb的粘质沙雷氏菌DNA。将任一质粒导入大肠杆菌都会导致溶血素产量显著增加，同时细胞活力下降。
